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- 저자 : Schneewind, Olaf (검색결과 1 건)
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Cambronne, Eric D.,Schneewind, Olaf American Society for Microbiology 2002 Journal of Bacteriology Vol.184 No.21
<B>ABSTRACT</B><P>Pathogenic <I>Yersinia</I> spp. secrete Yops (<I>Yersinia</I> outer proteins) via the type III pathway. The expression of <I>yop</I> genes is regulated in response to environmental cues, which results in a cascade of type III secretion reactions. <I>yscM1</I> and <I>yscM2</I> negatively regulate the expression of <I>Yersinia enterocolitica yop</I> genes. It is demonstrated that <I>yopD</I> and <I>lcrH</I> are required for <I>yscM1</I> and <I>yscM2</I> function and that all four genes act synergistically at the same regulatory step. Further, SycH binding to the protein products of <I>yscM1</I> and <I>yscM2</I> can activate <I>yop</I> gene expression even without promoting type III transport of YscM1 and YscM2. Reverse transcription-PCR analysis of <I>yopQ</I> mRNA as well as <I>yopQ</I> and <I>yopE</I> gene fusion experiments with the <I>npt</I> (neomycin phosphotransferase) reporter suggest that <I>yscM1</I> and <I>yscM2</I> regulate expression at a posttranscriptional step. The 178-nucleotide 5′ untranslated region (UTR) of <I>yopQ</I> mRNA was sufficient to confer <I>yscM1</I> and <I>yscM2</I>-mediated regulation on the fused reporter, as was the 28-nucleotide UTR of <I>yopE</I>. The sequence 5′-AUAAA-3′ is located in the 5′ <I>yop</I> UTRs, and mutations that alter the sequence motif either reduced or abolished <I>yscM1-</I> and <I>yscM2</I>-mediated regulation. A model is proposed whereby YopD, LcrH, YscM1, YscM2, and SycH regulate <I>yop</I> expression in response to specific environmental cues and by a mechanism that may involve binding of some of these factors to a specific target sequence within the UTR of <I>yop</I> mRNAs.</P>
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