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Mustapha Goni Abatcha,Pei Ling Tan,Li- Oon Chuah,Gulam Rusul,S. R. Chandraprasad,Mohd. Esah Effarizah 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.8
The effectiveness of two different rapid methodsinvolving the 3MTM molecular detection assay Listeria andthe 3MTM Petrifilm environmental Listeria Plate wereevaluated for the rapid detection of Listeria from naturallycontaminated vegetables and chicken-processing environmentsagainst the standard culture-based method. A total of178 samples were examined for the presence of Listeria. Atotal of 47/178 (26.4%) by standard ISO culture-basedmethod (EN ISO 11290-1), 42/178 (23.6%) by 3MTMMDA Listeria and 40/178 (22.5%) by 3MTM Petrifilm ELPlate showed positive results, respectively. The accuracy,sensitivity, specificity, positive predictive value, and negativepredictive value for 3MTM MDA Listeria and 3MTMPetrifilm EL Plate were 97.2, 89.4, 99.3, 97.7, 96.4% and96.1, 85.1, 100.0, 100.0, 94.9%, respectively. Based on theCohen’s Kappa value, there was a complete and robustconcordance between 3MTM MDA Listeria (0.911) and3MTM Petrifilm EL Plates (0.894) as compared to thestandard culture-based method.