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RISS 인기검색어
- 검색키워드
- 저자 : Ranjeet Kumar (검색결과 4 건)
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Role of Arbitrary Intensity Profile Laser Beam in Trapping of RBC for Phase-imaging
Ranjeet Kumar,Vishal Srivastava,Dalip Singh Mehta,Chandra Shakher 한국광학회 2016 Current Optics and Photonics Vol.20 No.1
Red blood cells (RBCs) are customarily adhered to a bio-functionalised substrate to make them stationaryin interferometric phase-imaging modalities. This can make them susceptible to receive alterations in innatemorphology due to their own weight. Optical tweezers (OTs) often driven by Gaussian profile of a laserbeam is an alternative modality to overcome contact-induced perturbation but at the same time a steeplyfocused laser beam might cause photo-damage. In order to address both the photo-damage and substrateadherence induced perturbations, we were motivated to stabilize the RBC in OTs by utilizing a laser beamof ‘arbitrary intensity profile’ generated by a source having cavity imperfections per se. Thus the immobilizedRBC was investigated for phase-imaging with sinusoidal interferograms generated by a compact and robustMichelson interferometer which was designed from a cubic beam splitter having one surface coated withreflective material and another adjacent coplanar surface aligned against a mirror. Reflected interferogramsfrom bilayers membrane of a trapped RBC were recorded and analyzed. Our phase-imaging set-up is limitedto work in reflection configuration only because of the availability of an upright microscope. Due to RBC’smembrane being poorly reflective for visible wavelengths, quantitative information in the signal is weakand therefore, the quality of experimental results is limited in comparison to results obtained in transmissionmode by various holographic techniques reported elsewhere.
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A Probabilistic-Based Reputation Computation Model for VANET
Kapil Sharma,Aditya shrivastava,Ranjeet Singh Tomar,Brijesh Kumar Chaurasia,Namkyun Baik 보안공학연구지원센터 2016 International Journal of Software Engineering and Vol.10 No.12
Reputation computation model facilitates vehicles to broadcast traffic information in a vehicular ad hoc network (VANET) in order to improve road safety and efficiency. In this paper, a probabilistic-based reputation computation model for VANETs has been evaluated. The uncertainty of reputation is transformed into a probability distribution over possible reputed states that are hidden from observation but determined by a vehicle’s expected experience in VANET are addressed in this work. HMM is utilized to evaluate the level of reputation in VANET exhibiting dynamic behaviour. The proposed mechanism is probability distribution centric, though the later mechanism is docile to predict accurate trust value of messages. The time taken by the proposed scheme is significantly less and transmission over the shared wireless channel is in the order of milliseconds. Extensive results and simulation show that efficacy of the proposed model.
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Vishal Srivastava,Mohammad Inam,Ranjeet Kumar,Dalip Singh Mehta 한국광학회 2016 Current Optics and Photonics Vol.20 No.6
We present a single shot low coherence white light Hilbert phase microscopy (WL-HPM) for quantitativephase imaging of Si opto-electronic devices, i.e., Si integrated circuits (Si-ICs) and Si solar cells. Whitelight interferograms were recorded by a color CCD camera and the interferogram is decomposed into thethree colors red, green and blue. Spatial carrier frequency of the WL interferogram was increasedsufficiently by means of introducing a tilt in the interferometer. Hilbert transform fringe analysis was usedto reconstruct the phase map for red, green and blue colors from the single interferogram. 3D step heightmap of Si-ICs and Si solar cells was reconstructed at multiple wavelengths from a single interferogram. Experimental results were compared with Atomic Force Microscopy and they were found to be close toeach other. The present technique is non-contact, full-field and fast for the determination of surfaceroughness variation and morphological features of the objects at multiple wavelengths.
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Exploration of Heat Stress-Responsive Markers in Understanding Trait Associations in Wheat
Manjunatha P. B.,Nivedita Sinha,Hari Krishna,Divya Chauhan,Pawan Kumar,Ranjeet R. Kumar,Neelu Jain,P. K. Singh,G. P. Singh 한국식물학회 2021 Journal of Plant Biology Vol.64 No.2
Heat stress (HS) is detrimental to wheat production and productivity globally. To combat HS, several genetic, molecular, and genomic approaches have been employed in the past. Analyzing the physiochemical mechanisms and the important regulatory genes involved is the key to develop HS tolerant plants. In the present work, a total of 243 novel simple sequence repeat (SSR) markers developed from stress-associated genes identified through RNA-seq were used for understanding marker–trait associations. 37 SSRs were found to be clearly polymorphic and among these, 28 SSR loci were significantly associated with component traits of HS tolerance. The polymorphic SSRs were validated for diversity analysis on a subset of 85 genotypes. The genotypes were grouped into four clusters representing diverse and similar alleles imparting HS tolerance in Indian and exotic genotypes. Additionally, 28 genes selected for the expression analysis confirmed that 15 genes were induced under HS in the thermotolerant WH1021 and Raj3765 and repressed in thermosusceptible HD2009 cultivar. Hence, the information on traits associated with candidate genes and the SSR markers overlying on the gene will enhance our understanding of thermotolerance mechanism operating in wheat and will help the breeders in the precise development of heat-tolerant genotypes through marker-assisted selection (MAS).
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