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Urban, Scott D.,Aram Elovic,Randy Todd,Gallagher, George T.,Donoff, R. Bruce,Wong, David T. W. Korean Academy of Oral Biology and the UCLA Dental 1998 International Journal of Oral Biology Vol.23 No.2
Malignant ameloblastoma is characterized by aggressive behavior and a poor prognosis. No histomorphologic criteria exist to differentiate malignant ameloblastoma from their benign, locally-aggressive counterparts. However, biological markers such as histone (H3) expression have proven to be an important indicator of bioaggressive potential and may be a useful predictor of disseminated ameloblastoma. We hypothesize that malignant ameloblastoma, while histologically identical to their benign counterparts, will have a demonstrably higher proliferation rate by histone (H3) mRNA labeling. Seven cases of formalin-fixed, paraffin-embedded tissues from surgical biopsies of mandibular follicular ameloblastomas were obtained. One of the seven specimens was obtained from an ameloblastoma which metastasized to the right and left lungs. Proliferation rates were determined using RNA in situ hybridization with ^35S-labeled histone (H3) antisense and sense riboprobes. A blinded correlative assessment was made between the labeling index of H3 and the clinical outcome. The quantitative tumor labeling index of the malignant ameloblastoma (HLI=0.329) was higher than the indices of the benign, locally aggressive ameloblastomas (HLI=0.049 to 0.111). This preliminary examination suggests that the basis of the rare metastatic behavior of ameloblastoma is possibly a reflection of an elevated cellular proliferation rate.
Murine doc-1 cDNA Cloning, Sequencing and Expression in Normal Adult Tissues
Kim, Young,Tsuji, Takanori,Elovic, Aram,Shintani, Satoru,Mihara, Mariko,Salih, Erdjan,Kohno, Yohko,Chin, Byung-Rho,Patel, Vipul,Wong, David,Todd, Randy Korean Academy of Oral Biology and the UCLA Dental 2001 International Journal of Oral Biology Vol.26 No.3
Human Deleted in Oral Cancer-1 (DOC-1) is a 115-amino acid polypeptide (p12^DOC-1) that is widely expressed in normal adult tissues but markedly reduced or absent in oral squamous cell carcinomas. We isolated the murine doc-1 cDNA homologue from NIH3T3 cells using PCR and 3'RACE. Sequence analysis confirms an open reading frame of 114 amino acids, with a 100% and 98% homology with hamster p12^doc-1 and human p12^doc-1 respectively. Gene expression and cellular localization of doc-1 were demonstrated using northern analysis and in situ hybridization using several adult mouse tissues. The murine p12^doc-1 peptide sequence was confirmed using protein from mouse liver extract purified by a cation exchange column and fractionated by reverse-phase HPLC. Western analysis was performed to verify the presence of p12^doc-1 in adult murine tissues. The production and cellular localization of p12^doc-1 were confirmed by western analysis and immunohistochemistry in corresponding tissues. This is the first study to examine the cellular localization of doc-1 mPNA and p12^doc-1.