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Mahittikon Jenjuiree,Thitiprasert Sitanan,Thongchul Nuttha,Tanaka Naoto,Shiwa Yuh,Chamroensaksri Nitcha,Tanasupawat Somboon 한국미생물·생명공학회 2023 한국미생물·생명공학회지 Vol.51 No.4
This study aimed to isolate and identify L-(+)-lactic acid-producing bacteria from tree barks collected in Thailand and evaluate the potential strain as probiotics. Twelve strains were isolated and characterized phenotypically and genotypically. The strains exhibited a rod-shaped morphology, high-temperature tolerance, and the ability to ferment different sugars into lactic acid. Based on 16S rRNA gene analysis, all strains were identified as belonging to Weizmannia coagulans. Among the isolated strains, BKMTCR2-2 demonstrated exceptional lactic acid production, with 96.41% optical purity, 2.33 g/l of lactic acid production, 1.44 g/g of lactic acid yield (per gram of glucose consumption), and 0.0049 g/l/h of lactic acid productivity. This strain also displayed a wide range of pH tolerance, suggesting suitability for the human gastrointestinal tract and potential probiotic applications. The whole-genome sequence of BKMTCR2-2 was assembled using a hybridization approach that combined long and short reads. The genomic analysis confirmed its identification as W. coagulans and safety assessments revealed its non-pathogenic attribute compared to type strains and commercial probiotic strains. Furthermore, this strain exhibited resilience to acidic and bile conditions, along with the presence of potential probiotic-related genes and metabolic capabilities. These findings suggest that BKMTCR2-2 holds promise as a safe and effective probiotic strain with significant lactic acid production capabilities.
Tanatorn Saisavoey,Tanapat Palaga,Suchinda Malaivijitnond,Sukanya Jaroenporn,Nuttha Thongchul,Polkit Sangvanich,Aphichart Karnchanatat 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
The flavonoid contents of intact tubers and cellculture media were determined and physiological activitiesof Pueraria mirifica extracts were investigated. The totalflavonoid contents from cell culture media (PMC) werehigher than from tubers (PMT). Results from in vivoestrogenic activity assays indicated that PMT had a strongestrogenic activity in ovariectomized rats. The same amountof PMC exhibited a weak activity. In vitro osteoclastsuppression investigations indicated that both PMT andPMC extracts exhibited anti-osteoclastogenic activities withlow toxicities in a standard test cell line. Determination ofthe antioxidant potential using the DPPH assay revealedthat the IC50 value for PMT was lower than for PMC. P. mirifica cell cultures produce more flavonoids and exhibita mild estrogenic and more antioxidant activities than tubers.