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RISS 인기검색어
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- 저자 : Nurulita Nunuk Aries (검색결과 2 건)
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Nurulita, Nunuk Aries,Meiyanto, Edy,Sugiyanto, Sugiyanto,Matsuda, Eishou,Kawaichi, Masashi 경희한의학연구센터 2012 Oriental Pharmacy and Experimental Medicine Vol.12 No.3
Recent studies both in vitro and in vivo of G. procumbens exhibits chemopreventive properties for tumor inhibition on several types of cancer. Our study was carried out to observe the anticancer property of ethyl acetate fraction of G. procumbens leaves (FEG) on breast cancer cells as well as the co-chemotherapeutic potential, and to investigate its molecular mechanisms. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the growth inhibitory effect of FEG, doxorubicin (DOX), and 5-fluorouracil (5-FU) and their combination. Flowcytometry, 4',6-diamidino-2-phenylindole (DAPI) staining, and immunobloting were used to explore the mechanism of cell cycle arrest and apoptosis. FEG inhibited cell proliferation, induced G1 phase arrest and apoptosis. The inhibitory effect of FEG was enhanced when combined with Dox and 5-FU. The apoptosis induction was related to the increase of c-PARP expression after combination treatment of FEG and Dox or 5-FU onMCF-7 cells. However, treatment of DOX, 5-FU, and FEG on T47D cells, resulting no significance DNA fragmentation and nuclei condensation evidance. Only combination treatment of 5-FU+FEG showed c-PARP expression in T47D cells. In T47D cells, The FEG treatment also caused the decrease of microtubule expression as shown by Western blotting assay. The decreasing level of microtubul expression might be caused by protein aggregation, as shown by immunostaning using ${\alpha}$-tubulin antibody. All these results suggest that FEG potentiates the DOX and 5-FU efficacy on MCF-7 and T47D cells. FEG induces T47D cell death through different mechanism than MCF-7 that proposed to be mitotic catastrophe. The FEG may have specific targeted on microtubule integrity modulation leading to the cell cycle arrest and proliferation inhibition. Further FEG could be developed as a co-chemotherapeutic agent for reducing side effect and have specific molecular target for breast cancer.
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Anti-aging activity of tetrahydrocurcumin, Centella asiatica extract, and its mixture
Astuti Ika Yuni,Yupitawati Ani,Nurulita Nunuk Aries 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.1
Tetrahydrocurcumin has potent antioxidant activity and depigmentation of the skin. Centella asiatica extract can stimulate the formation of collagen, thus reducing wrinkles in aging skin. The combination of tetrahydrocurcumin (THC) and Centella asiatica extract (CAE) is expected to improve various parameters of premature aging. This study used pre-test post-test control group design. Mice were randomized into 5 test groups. The dorsal part of the mice was shaved, and then various anti-aging parameters, including skin sensitivity, moisture content, collagen levels, elasticity, and large pores, were meas-ured using a skin analyzer. Furthermore, the dorsal was smeared with a cream base for the negative control (NC) group, a cream containing ethyl ascorbyl ether for the positive control (PC) group, a cream containing THC for THC group, a cream containing CAE for the CAE group, and a cream containing THC and CAE for the Combination group, each with a dose of 2 mg/cm2 before and after UV irradiation. After treatment for four weeks, the anti-aging parameters were measured again. The combination of THC and CAE could increase the collagen and elasticity levels compared to THC and CAE itself, but the increase was not significant. Based on all anti-aging parameters scores, THC and CAE’s combination did not provide more significant anti-aging activity than single THC and single CAE.
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