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RISS 인기검색어
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- 저자 : Mobley, Harry L.T. (검색결과 3 건)
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Lee, Jong Wha,Shin, Min Hyeon,Mobley, William,Urbach, Adam R.,Kim, Hugh I. American Chemical Society 2015 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.137 No.48
<P>Mass spectrometry (MS)-based analysis using enzymatic digestion is widely used for protein sequencing and characterization. The large number of peptides generated from proteolysis, however, suppresses the signal of peptides with low ionization efficiency, thus precluding their observation and analysis. This study describes a technique for improved analysis of peptic peptides by adding the synthetic receptor cucurbit[7]uril (CB[7]), which binds selectively to peptides with N-terminal aromatic residues. Capturing the N-terminal phenylalanine (Phe) of peptides using CB[7] enhances the peptide abundances both in electrospray ionization MS and in matrix-assisted laser desorption ionization MS. Moreover, collision-induced dissociation (CID) of the CB[7]·peptide complex ions generates b- and y-type fragment ions with higher sequence coverage than those generated with uncomplexed peptides. The signal enhancement mediated by CB[7] is attributed to an increase in the peptide proton affinities upon CB[7] complexation. The mechanistic details of the fragmentation process are discussed on the basis of the structures of the complex ions obtained from ion mobility (IM) measurements and molecular modeling. This study demonstrates a novel and powerful approach to the enhancement of protein and peptide analysis using a synthetic receptor, without the need for new instrumentation, chemical modifications, or specialized sample preparation. The simplicity and potential generality of this technique should provide a valuable asset in the toolbox of routine protein and peptide analysis.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2015/jacsat.2015.137.issue-48/jacs.5b10648/production/images/medium/ja-2015-10648e_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja5b10648'>ACS Electronic Supporting Info</A></P>
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Lee, ZhongPing,Ahn, Yu-Hwan,Mobley, Curtis,Arnone, Robert The Optical Society 2010 Optics express Vol.18 No.25
<P>Using hyperspectral measurements made in the field, we show that the effective sea-surface reflectance ? (defined as the ratio of the surface-reflected radiance at the specular direction corresponding to the downwelling sky radiance from one direction) varies not only for different measurement scans, but also can differ by a factor of 8 between 400 nm and 800 nm for the same scan. This means that the derived water-leaving radiance (or remote-sensing reflectance) can be highly inaccurate if a spectrally constant ? value is applied (although errors can be reduced by carefully filtering measured raw data). To remove surface-reflected light in field measurements of remote sensing reflectance, a spectral optimization approach was applied, with results compared with those from remote-sensing models and from direct measurements. The agreement from different determinations suggests that reasonable results for remote sensing reflectance of clear blue water to turbid brown water are obtainable from above-surface measurements, even under conditions of high waves.</P>
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Seo, S.U.,Kamada, N.,Munoz-Planillo, R.,Kim, Y.G.,Kim, D.,Koizumi, Y.,Hasegawa, M.,Himpsl, Stephanie D.,Browne, Hilary P.,Lawley, Trevor D.,Mobley, Harry L.T.,Inohara, N.,Nunez, G. Cell Press 2015 Immunity Vol.42 No.4
The microbiota stimulates inflammation, but the signaling pathways and the members of the microbiota involved remain poorly understood. We found that the microbiota induces interleukin-1β (IL-1β) release upon intestinal injury and that this is mediated via the NLRP3 inflammasome. Enterobacteriaceae and in particular the pathobiont Proteus mirabilis, induced robust IL-1β release that was comparable to that induced by the pathogen Salmonella. Upon epithelial injury, production of IL-1β in the intestine was largely mediated by intestinal Ly6C<SUP>high</SUP> monocytes, required chemokine receptor CCR2 and was abolished by deletion of IL-1β in CCR2<SUP>+</SUP> blood monocytes. Furthermore, colonization with P. mirabilis promoted intestinal inflammation upon intestinal injury via the production of hemolysin, which required NLRP3 and IL-1 receptor signaling in vivo. Thus, upon intestinal injury, selective members of the microbiota stimulate newly recruited monocytes to induce NLRP3-dependent IL-1β release, which promotes inflammation in the intestine.
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