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RISS 인기검색어
- 검색키워드
- 저자 : Melencion, A. (검색결과 2 건)
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Melencion, Sarah Mae Boyles,Chi, Yong Hun,Pham, Thuy Thi,Paeng, Seol Ki,Wi, Seong Dong,Lee, Changyu,Ryu, Seoung Woo,Koo, Sung Sun,Lee, Sang Yeol MDPI AG 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12
<P>The physiological function of <I>Arabidopsis thaliana</I> universal stress protein (AtUSP) in plant has remained unclear. Thus, we report here the functional role of the <I>Arabidopsis</I> universal stress protein, AtUSP (At3g53990). To determine how AtUSP affects physiological responses towards cold stress, AtUSP overexpression (AtUSP OE) and T-DNA insertion knock-out (<I>atusp</I>, SALK_146059) mutant lines were used. The results indicated that AtUSP OE enhanced plant tolerance to cold stress, whereas <I>atusp</I> did not. AtUSP is localized in the nucleus and cytoplasm, and cold stress significantly affects RNA metabolism such as by misfolding and secondary structure changes of RNA. Therefore, we investigated the relationship of AtUSP with RNA metabolism. We found that AtUSP can bind nucleic acids, including single- and double-stranded DNA and luciferase mRNA. AtUSP also displayed strong nucleic acid-melting activity. We expressed AtUSP in RL211 <I>Escherichia coli</I>, which contains a hairpin-loop RNA structure upstream of chloramphenicol acetyltransferase (<I>CAT</I>), and observed that AtUSP exhibited anti-termination activity that enabled <I>CAT</I> gene expression. AtUSP expression in the cold-sensitive <I>Escherichia coli (E. coli)</I> mutant BX04 complemented the cold sensitivity of the mutant cells. As these properties are typical characteristics of RNA chaperones, we conclude that AtUSP functions as a RNA chaperone under cold-shock conditions. Thus, the enhanced tolerance of AtUSP OE lines to cold stress is mediated by the RNA chaperone function of AtUSP.</P>
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Cha, J.Y.,Kim, J.Y.,Jung, I.J.,Kim, M.R.,Melencion, A.,Alam, S.S.,Yun, D.J.,Lee, S.Y.,Kim, M.G.,Kim, W.Y. Gauthier-Villars ; Elsevier Science Ltd 2014 Vol. No.
<P>NADPH-dependent thioredoxin reductases (NTRs) are key-regulatory enzymes determining the redox state of the thioredoxin (Trx) system that provides reducing power to peroxidases or oxidoreductases. Moreover, it also plays an essential function in the direct reduction of ROS and acquiring stress tolerance in plant. Cytoplasmic NTRA, mitochondrial NTRB, and chloroplastic NTRC are the three conserved NTRs which cooperate with specific sub-cellularly localized Trxs in Arabidopsis. However, cytosolic NTRs such as NTRA in Arabidopsis have not previously been identified in plants or mammals as a source of functional redundancy with mitochondrial NTRs. Here, we show the involvement of NTRA in the plant stress response counteracting oxidative and drought stresses. Methyl viologen (MV), an inducer of oxidative stress in plants, enhanced the NTRA transcripts. To identify the physiological role of NTRA influencing ROS homeostasis by stress, NTRA overexpression (NTRAOX) and knock-out mutants (ntra-ko) were generated. After exposure to oxidative stress, wild-type and ntra-ko plants were sensitive, but NTRAOX plants tolerant. ROS range was increased by MV in wild-type and ntra-ko plants, but not in NTRAOX. Investigating the involvement of Arabidopsis NTRA in drought, NTRAOX plants exhibited extreme drought tolerance with high survival rates, lower water loss and reduced ROS compared to wild-type and ntra-ko plants. Transcripts of drought-responsive genes, such as RD29A and DREB2A, were highly expressed under drought and antioxidant genes, namely CuZnSOD and APX1 were enhanced in the absence of drought in NTRAOX plants. The results suggest that NTRA overexpression confers oxidative and drought tolerance by regulation of ROS amounts. (C) 2014 Elsevier Masson SAS. All rights reserved.</P>
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