http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Role of platelet CD40 ligand for endothelial cell-monocyte interaction in the presence of flow
Andreas H. Wagner,Manuel Schwarz,Gerd König,Markus Hecker 한국유변학회 2014 Korea-Australia rheology journal Vol.26 No.4
CD40 ligand (CD154)-induced ultra-large von Willebrand factor (vWF) multimer-mediated endothelial cellplatelet-monocyte interaction may play an important role in adaptive and maladaptive vascular remodelingprocesses. Here we analyzed the impact of and conditions favouring the deposition of these multimers onthe endothelial cell (EC) surface by way of CD40-CD154 co-stimulation in settings mimicking differentforms of blood flow. Upon exposure to low oscillatory shear stress and sCD154, a release of vWF multimerscomparable to histamine stimulation was monitored on the EC surface in a string-like fashion. Moreover,ex vivo perfused carotid arteries of wild type mice at low laminar shear stress rates showed a luminalrelease of vWF as ultra-large vWF multimers (ULVWF) upon stimulation with sCD154 which was absentin blood vessels of CD40 knockout mice. The observed CD40- and flow-dependent vWF release from intactendothelial cells and subsequent vWF multimer formation may facilitate adhesion and subsequent activationof circulating platelets at atherosclerotic predilection sites, which are characterized by disturbed flow patterns. This in turn may amplify endothelial cell-monocyte interaction, thus possibly initiating or promotingearly atherosclerotic lesion formation.