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RISS 인기검색어
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- 저자 : Lianji Jin (검색결과 4 건)
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Tian, Lianji,Kim, Hoe Suk,Kim, Heyonjin,Jin, Xing,Jung, Hye Seung,Park, Kyong Soo,Cho, Kyoung Won,Park, Sunghyouk,Moon, Woo Kyung American Chemical Society 2013 Journal of proteome research Vol.12 No.8
<P>This study was designed to investigate changes in the metabolites in the intracellular fluid of the pancreatic β-cell line INS-1 to identify potential early and late biomarkers for predicting hypoxia-induced cell death. INS-1 cells were incubated under normoxic conditions (95% air, 5% CO<SUB>2</SUB>) or hypoxic conditions (1% O<SUB>2</SUB>, 5% CO<SUB>2</SUB>, 95% N<SUB>2</SUB>) for 2, 4, 6, 12, or 24 h. The biological changes indicating the process of cell death were analyzed using the MTT assay, flow cytometry, Western blotting, and immunostaining. Changes in the metabolic profiles from cell lysates were identified using <SUP>1</SUP>H nuclear magnetic resonance (<SUP>1</SUP>H NMR) spectroscopy, and the spectra were analyzed by the multivariate model Orthogonal Projections to Latent Structure-Discriminant Analysis. Cell viability decreased approximately 40% after 12–24 h of hypoxia, coincident with a high level of cleaved caspase-3. A high level of HIF-1α was detected in the 12–24 h hypoxic conditions. The metabolite profiles were altered according to the degree of exposure to hypoxia. A spectral analysis showed significant differences in creatine-containing compounds at the early stage (2–6 h) and taurine-containing compounds at the late stage (12–24 h), with the detection of HIF-1α and cleaved caspase-3 in cells exposed to hypoxia compared to normoxia. Glycerophosphocholine decreased during the early stage hypoxia. The change in taurine- and creatine-containing compounds and choline species could be involved in the β-cell death process as inhibitors or activators of cell death. Our results imply that assessment by <SUP>1</SUP>H NMR spectroscopy would be a useful tool to predict the cell death process and to identify molecules regulating hypoxia-induced cell death mechanisms.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2013/jprobs.2013.12.issue-8/pr400315e/production/images/medium/pr-2013-00315e_0007.gif'></P>
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조윤호(Younho Cho),LIANJI JIN,최영권(Youngkwon Choi) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5
Mode conversion was studied with thin plate defects by using lamb wave scattering analysis. Phase velocity dispersion curve and group velocity dispersion curve of material were calculated for lamb wave scattering analysis. A defect shape was obtained from boundary element method (B.E.M) with respect to reflection and transmission factor. Time domain wave analysis was also performed with scattering field modeling and inverse fast fourier transform (IFFT). A feasibility of lamb wave technique was discussed for various practical application including defect classification.
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Yunri Piao,조윤호(Younho Cho),Lianji Jin,안봉영(Bong-Young Ahn),김노유(Nohyu Kim),조승현(Seung Hyun Cho) 한국비파괴검사학회 2009 한국비파괴검사학회지 Vol.29 No.4
토모그래피는 다중 빔을 이용하여 단면을 영상화하는 기법으로서 주로 의료진단 분야에서 인체의 단면 영상획득을 위해 응용되어지는 기법이다. 비파괴검사 분야에서도 단순한 시간영역 신호의 제시에서 탈피하여 검사자에게 영상을 제공함으로써 진단의 효율성을 높이고자 하는 추세이므로 이 기법은 많은 의미를 갖는다. 최근, 유도초음파를 이용한 평판 구조물의 진단 기법이 많은 주목을 받고 있어, 본 논문에서는 컴퓨터 기반 유도초음파 해석 기법과 토모그래피 영상화 기법을 기반으로 2차원 평판에 존재하는 결함 위치를 영상화하는 연구를 수행하였다. 이를 위해 경계요소법을 이용하여 판 구조물에 존재하는 결함이 유도초음파의 전파 양상에 미치는 영향을 해석하고 그 결과를 토모그래피 영상화 기법에 적용하여 평판의 결함 위치를 판별하고자 하였다. 그 결과, 토모그래피를 위해 사용되는 센서의 개수가 결함 검출 성능에 많은 영향을 미침을 확인할 수 있다. Tomography is the imaging method of cross sectional area using multi beam signals and is mainly applied to the medical diagnosis to acquire the image of the inside human body. This method is pretty meaningful in nondestructive evaluation field since the imaging of the inspection region can enhance the comprehension of the inspector. Recently, much attention has been paid to the guided wave for the diagnosis of platelike structures. So, in this work, a study on the imaging of the damage location in a plate was carried out on the basis of computer aided analysis of guided waves and tomographic imaging. To this end, boundary element method was employed to analyze the effect of the damage in plate on the propagation of the guided waves and the analytic results were applied to the tomographic imaging method to identify the damage location. Consequently, it was shown that the number of sensors heavily affect the inspection performance of the damage location.
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실시간 진단을 위한 산재형 센서네트워크의 토모그래피 연구
조윤호(Younho Cho),박운일(Yunri Piao),신봉재(Bongjae Sheen),김련희(Lianji Jin),안봉영(Bongyoung Ahn),조승현(Seunghyun Cho) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.5
There has been much interest recently about the on-line structural health monitoring in plate-like structures using guided wave. The guided wave mode conversion phenomenon under through transmission scheme is simulated by the hybrid BEM and employed as a useful feature for defect location. The work presented here introduces a Reconstruction Algorithm for the Probabilistic Inspection of Damage (RAPID) which is used to construct tomographic images of defect. Through this study, we found a feasibility of defect image reconstruction based on the BEM modeling ad the tomograpy of guided waves.
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