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Kyungmok Lee(이경목),Denver Walitang,Tongmin Sa(사동민) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11
Plant growth promoting bacteria (PGPB) that can colonize the rhizosphere, endosphere, and phyllosphere of crops can help to enhance plant growth, nutrient mobilization, yield, and tolerance to biotic and abiotic stress. The increase in global population to an estimated 9.7 billion by 2050 necessitates the increase in food production and consumption. This increases our reliance to conventional yet unsustainable practices. The use of microbials as substitute for chemical fertilizers is an ecofriendly and sustainable way of increasing crop yield with lesser impact to the environment in support to the provisions of the Paris Climate Change Accord. PGPB with endophytic ability could colonize internal plant tissues and provide direct beneficial effects to the plants. The use of GPF tagging has been proven as a meaningful molecular confirmation to evaluate internal colonization of bacteria in the plant tissues. This study aims to evaluate endophytic colonization of two PGPB Pseudomonas strains and assess their plant growth promotion on onion, green onion and zucchini. For endophytic colonization, Pseudomonas frederiksbergensis OS261 and Pseudomonas vancouverensis OB155 were gfp-tagged through triparental mating with the donor E. coli strain pFAJ1820 and helper E. coli strain pRK2013. Confocal microscopy was used to evaluate the colonization and intercellular localization of the bacteria. We observed that Pseudomonas OB155 and OS261 successfully colonized the plant tissues. To evaluate the plant growth promotion of Pseudomonas OB155 and OS261 wild type, seeds of green onion, onion and zucchini were seed bacterized and grown in growth pouches under gnotobiotic conditions in plant growth chamber. Pseudomonas OB155 and OS261 have beneficial traits such as indole acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, salicyclic acid, phosphate solublization. Bacterial inoculated seeds had enhanced plant growth particularly root length and dry weight. The seeds which were inoculated with high indole acetic acid (IAA) producing bacteria, showed beneficial effect on plant growth during early seedling stage.