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Discrimination and Detection of Erwinia amylovora and Erwinia pyrifoliae with a Single Primer Set
함현희(Hyeonheui Ham),Kyongnim Kim,양수인(Suin Yang),공현기(Hyun Gi Kong),이미현(Mi-Hyun Lee),Yong Ju Jin,박동석(Dong Suk Park) 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.3
Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 104 cfu/ml and 107 cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms.