Post-consumer energy consumption of textile products during ‘use’ phase of the lifecycle

Kim, Jooyoun,Yun, Changsang,Park, Yaewon,Park, Chung Hee 한국섬유공학회 2015 Fibers and polymers Vol.16 No.4

Contribution of surface energy and roughness to the wettability of polyamide 6 and polypropylene film in the plasma-induced process

Kim, Jooyoun,Kim, Hey-sang,Park, Chung Hee Sage Science Press 2016 Textile Research Journal Vol. No.

<P>Plasma-induced etching and chemical vapor coating processes are well-known technologies that modify the wetting properties of polymeric surfaces by engineering the surface roughness and surface energy. The contributing effect of plasma treatment for O-2 etching and hexamethyldisiloxane (HMDSO) vapor coating on the wetting properties was investigated for polyamide 6 (PA6) and polypropylene (PP) substrates. The surface energy components of PA6 and PP were analyzed by the Owens-Wendt model, and were associated with the wettability of water and diiodomethane. With the introduction of roughness by the O-2 etching process without HMDSO coating, the wettability of PA6 substrate was enhanced while that of PP was deteriorated when they were observed after 20 days aging. When the surface was etched for 7min or longer with the subsequent coating with HMDSO, both PA6 and PP lost hydrophilic property, giving water contact angle of 180 degrees. The wettability was examined for the varied treatment conditions and as a function of average nano-pillar length. This study helps better understand the interactions between the surface energy and roughness of polymeric materials, and their influence on surface wettability.</P>

Incorporation of STED technique into single-molecule spectroscopy to break the concentration limit of diffusing molecules in single-molecule detection

Kim, Namdoo,Kwon, Jiwoong,Lim, Youngbin,Kang, Jooyoun,Bae, Sohyeon,Kim, Seong Keun The Royal Society of Chemistry 2018 Chemical communications Vol.54 No.69

<P>By incorporating STED (stimulated emission depletion) nanoscopy into single-molecule spectroscopy, we demonstrate that the concentration limit imposed by optical diffraction can be overcome in diffusion-based single-molecule measurement. We showed that single-molecule detection is feasible at a concentration of 5 nM, which is 100-times higher than the limit of conventional single-molecule measurements.</P>

Algivirga pacifica gen. nov., sp. nov., a Novel Agar-Degrading Marine Bacterium of the Family Flammeovirgaceae Isolated from Micronesia

Kim, Jennifer Jooyoun,Kim, Ji Hyung,Kwon, Young-Kyung,Kwon, Kae Kyoung,Yang, Sung-Hyun,Jang, Jiyi,Heo, Soo-Jin,Park, Heung-Sik,Jung, Won-Kyo,Lee, Youngdeuk,Kang, Do-Hyung,Oh, Chulhong Springer-Verlag 2013 Current microbiology Vol.67 No.6

일본어 모어 학습자의 한국어 평서문의 억양 분석 연구

김주연 ( Kim¸ Jooyoun ),김한샘 ( Kim Hansaem ) 연세대학교 언어정보연구원 2021 언어사실과 관점 Vol.54 No.-

This paper compares and analyzes the Korean intonation produced by native Korean speakers and Japanese Korean learners by examining the intonation acquisition patterns of Japanese Korean learners according to their proficiency in Korean. For this purpose, the intonation phrases in which the first syllable begins with a high tone and intonation phrases where the first syllable begins with a low tone were examined focusing on utterances of the plain text. Both the intonation phrase tone type and the boundary tone realization pattern showed that as Korean learning progressed from beginner to advanced, they became closer to the native speaker of the Korean language. Looking at the acquisition process, the method of realizing intonation phrases in Japanese, the learner’s native language, was transferred from the beginner stage, showing an intonation phrase and tone type that is not seen in Korean. In addition, there were errors in pronunciation of segmental consonants that were not found in the learner’s native language, and errors in pronunciation caused by not fully acquiring the phonological phenomenon of Korean. In addition, the transfer of the Japanese articulation method, which is the learner’s mother tongue, in the articulation method of segmental sounds had an effect on the length of the syllables, but showed a decreasing trend toward the intermediate level. From the above content, it can be confirmed that the intonation of the learner’s mother tongue and the articulation method of the segmental consonants have a considerable influence on the realization of intonation of Japanese learners. Therefore, it is necessary to explicitly include phonetic and phonological knowledge about the differences and commonalities between Korean and the learner’s native language from the beginner level segmental consonant education.

국어교과통합 진로탐색 프로그램이 중학생의 진로결정 자기효능감과 역기능적 진로 사고에 미치는 효과

김주연(Jooyoun Kim),김수리(Suri Kim) 글로벌지식융합학회 2023 지식융합연구 Vol.6 No.1

본 연구는 국어교과통합 진로교육 프로그램을 구안하여 학생들이 국어수업 내용과 자신의 꿈을 키워나갈 수 있는 진로 교육을 융합함으로써 학생들의 진로결정 자기효능감과 진로 사고에 어떤 영향을 미치는지 알아보는 데 목적이 있다. 연구 방법은 다음과 같다. 첫째, 중학교 3학년을 대상으로 국어교과통합 진로교육의 필요성을 고찰하였다. 둘째, 국어교과 성취기준과 진로연계 학습 요소를 융합하여 국어교과통합 진로교육 프로그램을 구안하였다. 셋째, 서울 소재 중학교 3학년 학생 127명 중 63명의 실험집단을 대상으로 국어교과통합 진로교육 프로그램을 8차시 적용하였다. 진로결정 자기효능감 검사 도구는 Taylor와 Betz(1983)가 개발한 진로결정 자기효능감 척도(Career Decision-Making Self-Efficacy Scale-SF)를 이은경(2001)이 중·고등학생들을 대상으로 타당화 작업한 것을 사용하였다. 진로결정 자기 효능감 척도는 목표선택, 직업정보, 문제해결, 미래계획 등 4개 하위요인의 총 25문항으로 구성되어 있다(이은경, 2001). 진로사고 검사(Career Thought Inventory: CTI) 도구는 Sampson 등(1996)이 개발하고 장선철(2003)이 요인분석하여 타당화한 검사 도구를 사용하였다. 이 검사지는 진로와 관련된 역기능적 진로 사고를 측정하는 것으로 진로 결정 과정에 관한 일반화된 의사결정 혼란, 수행불안, 왜곡된 정체감 등 3개 하위요인의 총 26문항으로 구성되어 있다. 수집된 자료는 SPSS 25.0을 사용하여 독립표본 t-test를 통해 두 집단 간 동질성 검증을 하였고, 이후 대응표본 t-test(Paired samples t-test)를 실시하였다. 그 결과 실험집단의 진로결정 자기효능감 전체 사전 평균점수는 84.35점, 사후 평균점수는 98.90점으로 국어교과통합 진로교육 프로그램을 실시하기 전보다 실시한 후 진로결정 자기효능감이 높아졌으며, 통계적으로 유의미한 차이를 보였다(t=-7.644, p<.001). 또한 실험집단의 진로사고검사의 전체 사전 평균점수는 54.21점, 사후 평균점수는 46.84점으로 국어교과통합 진로교육 프로그램을 실시하기 전보다 실시한 후 역기능적 진로 사고의 수준이 낮아졌으며, 통계적으로 유의미한 차이를 보였다(t=3.430, p<.01). 이와 같이 실험집단에서 국어교과통합 진로교육 프로그램을 실시하기 전보다 실시한 후에 진로결정 자기효능감 전체가 향상되고, 역기능적 진로 사고가 감소한 것으로 확인하였다. 본 연구는 다음과 같은 의의를 지닌다. 첫째 국어교과통합 진로교육 프로그램을 통해 학생의 진로결정 자기효능감을 향상시켰다. 둘째, 학생들이 진로탐색을 하는 데 있어 방해요소가 되는 역기능적 진로 사고를 감소시켰다. The purpose of this study is to find out how it affects students’ career decision-making self-efficacy and career thinking by devising a career education program that integrates Korean language subjects and integrating the contents of Korean language classes with career education that enables students to develop their dreams. there is. The research method is as follows. First, the need for career education integrated with Korean subjects was examined for the third year of middle school. Second, a career education program integrated with Korean language subjects was conceived by converging Korean language subject achievement standards and career-related learning elements. Third, the Korean language integrated career education program was applied to the 8th class for an experimental group of 63 out of 127 third-year middle school students in Seoul. As a career decision self-efficacy test tool, the Career Decision-Making Self-Efficacy Scale-SF developed by Taylor and Betz (1983) was validated by Lee Eunkyung(2001) for middle and high school students. used The self-efficacy scale for career decision-making consists of a total of 25 questions of 4 sub-factors: goal selection, job information, problem solving, and future planning (Lee Eunkyung 2001:49). The Career Thought Inventory (CTI) tool developed by Sampson et al. (1996) and validated by factor analysis by Jang Seon-Cheol(2003) was used. This questionnaire measures career-related dysfunctional career thinking and consists of a total of 26 questions in three sub-factors: generalized decision-making confusion, performance anxiety, and distorted identity regarding the career decision-making process. The collected data was verified for homogeneity between the two groups through an independent sample t-test using SPSS 25.0, followed by a paired samples t-test. As a result, the experimental group's total pre-average score for career decision self-efficacy was 84.35 points, and the post-average average score was 98.90 points. (t=-7.644, p<.001). In addition, the overall mean score of the career thinking test in the experimental group was 54.21 points, and the mean post-test score was 46.84 points. (t=3.430, p<.01). As such, it was confirmed that the overall self-efficacy in career decision-making improved and dysfunctional career thinking decreased after the implementation of the integrated Korean language career education program in the experimental group compared to before the implementation. This study has the following significance. First, the career decision-making self-efficacy of students was improved through the career education program integrated with Korean subjects. Second, it reduced the dysfunctional career thinking that interferes with students’ career exploration.

Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application

( Jennifer Jooyoun Kim ),( Young Kyung Kwon ),( Ji Hyung Kim ),( Soo Jin Heo ),( Young Deuk Lee ),( Su Jin Lee ),( Won Bo Shim ),( Won Kyo Jung ),( Jung Ho Hyun ),( Kae Kyoung Kwon ),( Do Hyung Kang ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.11

Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a 200 μl total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at 100ºC for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.

Leucine-induced localization of Leucyl-tRNA synthetase in lysosome membrane

Choi, Hyosun,Son, Jung Bae,Kang, Jooyoun,Kwon, Jiwoong,Kim, Jong Hyun,Jung, Minkyo,Kim, Seong Keun,Kim, Sunghoon,Mun, Ji Young Academic Press 2017 Biochemical and biophysical research communication Vol. No.

<P><B>Abstract</B></P> <P>Leucyl-tRNA synthetase (LRS) plays major roles in providing leucine-tRNA and activating mechanistic target of rapamycin complex 1 (mTORC1) through intracellular leucine sensing. mTORC1 activated by amino acids affects the influence on physiology functions including cell proliferation, protein synthesis and autophagy in various organisms. Biochemical results demonstrating leucine sensing have been published, but visual results are lacking. Therefore, we observed the location of LRS with and without leucine using stimulated emission depletion (STED) microscopy one of the super-resolution microscopy and transmission electron microscopy (TEM). This revealed that LRS was translocated to the lysosome on addition of leucine. The translocation was inhibited by treatment with compound BC-LI-0186, disrupting the interaction between RagD and LRS. Immuno-TEM revealed a clear decrease in LRS translocation to the lysosome on addition of the inhibitor. This direct visualization of leucine sensing and LRS translocation to the lysosome was related to mTORC1 activation. To study the relationship between mTORC1 activation and LRS translocation, we monitored the change in autophagy for each condition using TEM and CLSM. The results showed a decrease in autophagy on addition of leucine, demonstrating crosstalk between leucine sensing, LRS translocation, RagD interaction, and mTORC1 activation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LRS activates mTORC1 via intracellular leucine sensing. </LI> <LI> We used STED microscopy and TEM to observe effect of leucine on LRS localization in lysosomes. </LI> <LI> LRS translocated to lysosomes and autophagy decreased after adding leucine. </LI> <LI> BC-LI-0186 inhibited translocation of LRS. </LI> <LI> LRS inhibition or LRS-mediated mTORC1 suppression can be a new therapeutic target. </LI> </UL> </P>

역전기투석 스택 구조에 따른 성능 평가

남주연(Jooyoun Nam),엄현지(Hyunji Eom),좌은진(Eunjin Jwa),황교식(Kyo Sik Hwang),정윤철(Yoon-Cheul Jeung),김한기(Hanki Kim),정남조(Namjo Jeong) 대한환경공학회 2022 대한환경공학회 학술발표논문집 Vol.2022 No.11

고효율 전력 생산을 위한 캐스케이드형 역전기투석 스택

남주연(Nam Jooyoun),좌은진(Eunjin Jwa),엄현지(Hyunji Eom),김한기(Hanki Kim),황교식(Kyosik Hwang),정남조(Namjo Jeong),정윤철(Yooncheol Jeung) 대한환경공학회 2021 대한환경공학회 학술발표논문집 Vol.2021 No.11