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Menglong Wang,Lan Jiang,Qingen Da,Jun Liu,Dongru Feng,Jinfa Wang,Hong-Bin Wang,Hong-Lei Jin 한국식물학회 2017 Journal of Plant Biology Vol.60 No.6
Plastid-encoded plastid RNA polymerase (PEP) isessential for chloroplast development and plastid geneexpression in Arabidopsis thaliana. However, PEP is a largecomplex, and many proteins in this complex remain to beidentified. We previously reported that Delayed Greening238 (DG238) interacts with PEP subunit protein FLN1 andmay function as a PEP-associated protein and participate inearly chloroplast development and PEP-dependent plastidgene expression. DG238 contains Domain of UnknownFunction 143 (DUF143), whose function is currently unknown. Here, we found that a deficiency of the DUF143 domain inDG238 affected its localization, which resulted in abnormalinteractions with PEP-associated proteins in the chloroplast. Furthermore, DG238 lacking the DUF143 domain or DG238with only this domain failed to function. Interestingly, thelack of conserved amino acids 193–217 of the DUF143domain in DG238 also affected its function. In addition toFLN1, DG238 also interacts with other PEP-associatedproteins, including FSD2, FSD3, MRL7-L, and MRL7, toregulate plastid gene expression. These results suggest thatthe DUF143 domain is necessary for the functioning of thePEP-associated protein DG238 in chloroplasts.
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Mengshu Li,Jie Zhang,Hong-Lei Jin,Dongru Feng,Jinfa Wang,Hong-Bin Wang,Bing Liu 한국식물학회 2019 Journal of Plant Biology Vol.62 No.2
In higher plants, iron (Fe) is an essential elementfor photosynthesis and growth. Two basic helix-loop-helix(bHLH) transcription factors, bHLH104 and IAA-LEUCINERESISTANT3 (ILR3), positively regulate Fe uptake inArabidopsis. Furthermore, an E3 ubiquitin ligase, BRUTUS(BTS) functions as a negative regulator upstream of bHLH104and ILR3 in the Fe homeostasis pathway. Interestingly, wecharacterized a lesion of BRUTUS (BTS), which exhibitedpale-green leaves and retarded in growth. Genetic analysesshowed that only loss of ILR3 could entirely compromise thechlorosis in bts-2 mutants. To further investigate whetherbHLH104 and ILR3 have different roles in functioningdownstream of BTS other than Fe absorption, we obtainedbHLH104-, and ILR3-overexpressing plants. Interestingly,similar to bts-2, overexpression of ILR3 displayed chloroticleaves. Moreover, overexpressing ILR3 also caused theabundance of thylakoid proteins reduced, along withphotosynthetic genes decreased. However, there was novisible difference between bHLH104-overexpressing plantsand WT. Furthermore, we found that bHLH104 and ILR3may act independently on different downstream targets. bHLH104, but not ILR3, could bind to the promoter of At-NEET, which acts as a Fe-S/Fe cluster donor in chloroplasts. Collectively, our data demonstrate that bHLH104 and ILR3possess different downstream targets that may have distincteffects on photosynthesis, although they share a commonfunction in Fe deficiency responses.
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