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Alginate Lyase Production of Halophilic Pseudomonas sp. by Recombinant Escherichia coli
Kong, In-Soo,Kim, Young-Ok,Kim, Jin-Man,Kim, Sung-Koo,Oh, Doo-Hwan,Yu, Ju-Hyun,Kong, Jal-Yul The Korean Society for Microbiology and Biotechnol 1995 Journal of microbiology and biotechnology Vol.5 No.2
Halophilic Pseudomonas sp.W7 isolated from laver in the southem sea of Korea showed alginate lyase activity. Gene (aly) encoding alginate lyase was cloned in E.coli JM83 and the N-terminal amino acid sequence of the enzyme was determined after purificaion. The recombinant enzyme has been shown to have a molecular weight of about 40kDa after 12% SDS-polyacrylamide gel electrophoresis.