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Mingle Wang,Jing Zhuang,Zhongwei Zou,Qinghui Li,Huahong Xin,Xinghui Li 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Dehydration-responsive element-binding protein(DREB) transcription factors play key roles in plant stresssignal transduction pathways. We herein describe the functionsof a Camellia sinensis DREB transcription factor (CsDREB)in response to abiotic stress. Subcellular localization analysesindicated that the CsDREB localizes to the nucleus. CsDREBexpression in C. sinensis leaves was induced by heat, cold,drought, high salinity, H2O2, and exogenous abscisic acid(ABA). Additionally, CsDREB showed no transcriptionalactivation in Saccharomyces cerevisiae. Transgenic Arabidopsisthaliana plants overexpressing CsDREB exhibited enhancedtolerance to salt and drought stresses. The overexpression ofCsDREB in A. thaliana plants resulted in the up-regulatedexpression of ABA-dependent stress-induced genes (i.e.,AtRD29B, AtRAB18, AtABI1, and AtABI2) and ABAindependentstress-induced genes (i.e., AtCOR15a andAtRD29A). Furthermore, an analysis of the CsDREB promotersequence revealed the presence of several abiotic and bioticstress-related motifs, along with the developmental stageandtissue-specific elements. An examination of thetransient expression of the CsDREB promoter in Nicotianabenthamiana leaves revealed that the promoter is highlyresponsive to ABA and methyl jasmonate. Collectively, theseresults suggest that CsDREB may increase plant tolerance tosalt and drought stresses via both ABA-dependent and ABAindependentpathways.