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RISS 인기검색어
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- 저자 : Garcia-Sierra, F. (검색결과 2 건)
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A Culture-Independent Comparison of Microbial Communities of Two Maturating Craft Beers Styles
Costa João,Sierra-Garcia Isabel N.,Cunha Angela 한국미생물·생명공학회 2022 한국미생물·생명공학회지 Vol.50 No.3
The process of manufacturing craft beer involves a wide variety of spontaneous microorganisms, acting in different stages of the brewing process, that contribute to the distinctive characteristics of each style. The objective of this work was to compare the structure of microbial communities associated with two different craft beer styles (Doppelbock and Märzen lagers), at a late maturation stage, and to identify discriminative, or style-specific taxa. Bacterial and fungal microbial communities were analyzed by Illumina sequencing of 16S rRNA gene of prokaryotes and the ITS 2 spacer of fungi (eukaryotes). Fungal communities in maturating beer were dominated by the yeast Dekkera, and by lactic acid (Lactobacillus and Pediococcus) and acetic acid (Acetobacter) bacteria. The Doppelbock barrels presented more rich and diverse fungal communities. The Märzen barrels were more variable in terms of structure and composition of fungal and bacterial communities, with occurrence of exclusive taxa of fungi (Aspergillus sp.) and bacteria (L. kimchicus). Minority bacterial taxa, differently represented in the microbiome of each barrel, may underlie the variability between barrels and ultimately, the distinctive traits of each style. The composition of the microbial communities indicates that in addition to differences related to upstream stages of the brewing process, the contact with the wood barrels may contribute to the definition of style-specific microbiological traits.
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One-dimensional ordered growth of magneto-crystalline and biocompatible cobalt ferrite nano-needles
Ravichandran, M.,Oza, G.,Velumani, S.,Ramirez, J.T.,Garcia-Sierra, F.,Andrade, N.B.,Garza-Navarro, M.A.,Garcia-Gutierrez, D.I.,Asomoza, R. North-Holland 2014 Materials letters Vol.135 No.-
<P>In this letter, we have reported a novel synthesis of CoFe2O4 nano-needles by a co-precipitation method using precursors of ferric chloride and cobalt nitrate at 80 degrees C. The structural and magnetic properties of as-grown needle-like CoFe2O4 nanostructures exhibit cubic spinel structure. CoFe2O4 nano-needles have an average diameter of 15 nm with an aspect ratio of 30:50, which was depicted by High-resolution transmission electron microscopy (HRTEM). Superconducting Quantum Interference Device (SQUID) confirmed the property of superparamagnetism. The magnetic measurement illustrated that the coercivity (Hc) of CoFe2O4 nano-needles increased from -145.84 Oe at 5 K to -25.38 Oe at 312 K. The cell viability studies of CoFe2O4 nano-needles were performed in the concentration range of 5-1000 mu g/ml on L6 (Skeletal muscle cell lines) and Hep-2 (Larynx carcinoma) cells using 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was more than 50% cell viability for both the cell lines, but Hep-2 cells were more prone to killing as compared to L6 cells. Such biocompatible nanostructures can be used as drug-delivery systems for cancer therapeutics. (C) 2014 Elsevier B.V. All rights reserved.</P>
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