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Chromosomal location of tillering gene in maize inbred line, Pl 213749
Hee Bong Lee(李喜鳳),E.H. Coe,Bong Ho Choe(崔鳳鎬) 한국육종학회 1996 한국육종학회지 Vol.28 No.2
In order of determine the chromosomal location of additional tillering gene, the PI213749 inbred was crossed with a non-tillered inbred, KV₁/IK₁, which has been selfed for six generations, and F₂ kernels were produced for RFLP mapping. Leaf tissue was collected from F₂ individuals and parents. After freeze drying, genomic DNA was extracted using the CTAB method of UMC Maize RFLP Laboratory Manual. DNA was digested with EcoRⅠ, HindⅢ and Dra I. DNA of the seperated parental lines, non-tiller and three or more tiller individuals, was loaded onto 0.8% agarose gels and electrophoresed. DNA was transferred to Magnacharge membrane according to standard laboratory protocols. Hybridization using dCTP³² oligolabelled probes from the Maize Core Marker Set indicated that tillering is linked to umc76 on the short arm of chromosome 1. Additional probings to establish whether the locus is proximal or distal to umc76 are in progress. Future testing will be needed to determine whether the gene from PI213749 and tlr I of Neuffer are allelic.
Hee Bong Lee(李喜鳳),Bong Ho Choe(崔鳳鎬),Won Koo Lee(李元九),E.H. Coe,Georgia Davis 한국육종학회 1995 한국육종학회지 Vol.27 No.2
In order to identify the location of rindless culm (rlc) gene derived from a Korean local maize, Dangjin line, homozygous rlc line was crossed with ten waxy-marked translocation sets. Ten sets of F₁ plant were selfed to produce F₂ seeds. F₂ kernels were separated into waxy and normal seed, and planted at each different row. Plants with rlc gene were counted in each row after tasseling. From Chi-square analysis, rlc gene was found to be very closely linked with T9-10b (p<0.005) which has a breakage point of 0.4 on short arm of chromosome 10. Morphological characteristics were observed in F₂ populations such as zigzag type, multi-tillers and ears and lower-node-eared plants.