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황석연 ( Seock Yeon Hwang ),김종석 ( Jong Seok Kim ),추상규 ( Sang Kyo Choo ),김일택 ( Il Teck Kim ),장철수 ( Cheoul Soo Chang ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
This study was carried out to evaluate the stability of commercial lyophllized control meterials being used for clinical chemical test and to investigate the substitutivity of a pooled serum prepared in our laboratory for those materials.Five sets of commercial lyophllized control meterials including normal and abnormal levels were obtained from five different manufacturers and stored under different conditions of storage(r∞m temp., refrigeration at 60C or freezing at 20C). πley were analyzed together with a pooled serum by Hitachl 7150 automated chemisσy analyzer and NOV A4 + electrolyte analyzer for the determination of 20 clinical chemical parameters and 3 electrolytes. The CV of within-batch, inter-batch and between-day in the precision analysis were below 1.3, 1.6 and 4.6%, respectively. It was revealed that total protein, albumin, creatinine, BUN, total cholesterol and TG were stable and their CV was less than 5% whlle total and direct bilirubin were unstable at room temperature and uφn light exposure with CV of 0.8 - 27.5 %. ALP at room temperature was also unstable. It was also revealed that pooled serum has no significant difference from those commercial control materials. The results above suggest that pooled serum could be used as a substitute for the commercial control materials with low cost.