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Chang Soon Ahn(安獎,淳),Kyung Soo Min(閔庚洙),Soon Ju Chung(鄭淳柱),Gap Cheon Koh(高甲千),Sok Hoon Park(朴錫勳) 한국육종학회 1986 한국육종학회지 Vol.18 No.4
Interspecific crosses between adzuki bean(Vigna angularis) and rice bean(V.umbellata) were made with six adzuki bean cultivars and five rice bean lines in order to determine the barriers to hybridization, and to assess the possibility of overcoming the barriers. There was apparently no pre-zygotic barrier between the two species as shown by normal pod set from the crosses in both directions. The pods, however, dried prematurely at early or later stage of development. Hybrid plants were secured in vitro culture of embryos from all the genotypic cross combinations of the two species, but only when rice bean was used as female parents. The F₁ plants were highly fertile to produce normal viable seeds from self pollination. Crossability of the interspecific derivatives with different local cultivars or lines of adzuki bean or rice bean clearly demonstrated that the major reproductive barrier between the species is the physiological post-zygotic embryo abortion and further genetic barriers, if present, may not be strong enough to completely restrict gene flow over species. Therfore, once hybrid plants secured by embryo culture, it would be highly possible to incorporate the desired characteristics of rice bean into adzuki bean cultivars by conventional breeding procedures.
Chang Soon Ahn(安獎,淳) 한국육종학회 1993 한국육종학회지 Vol.25 No.3
Seedlings of 5 crop species ; Lactuca sativa L., Capsicum annuum L., Cucumis sativus L., Lycopersicon esculentum Mill. and Sesamum indicum L. were inoculated with 6 wild strains of Agrobacterium rhizogenes, A4, A13, C8, M123, 1724 and 15834. Hairy roots were induced from almost all the combinations, though the plant species showed marked differences in the per cent hairy root formation. The root cultures derived from the initial hairy root tips displayed the typical hairy root phenotypes such as vigorous growth, diminished geotropism and abundant lateral roots. Hairy roots of cucumber, tomato and lettuce kept growing vigorously in subcultures, while the growth of those of hot pepper and sesame suspended in a few rounds of subcultures. Addition of GA₃ to the culture medium effectively accelerated the growth of hot pepper hairy roots. The formation of green and compact calli from the hairy roots cultured on the media containing NAA and zeatin indicated that plant regeneration would be readily induced by modification of culture medium or environmental parameters.