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- 저자 : Boussaoudi Ibtissam (검색결과 3 건)
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Conservation of Thymus pallidus Cosson ex Batt. by shoot tip and axillary bud in vitro culture
Ansari, Zineb Nejjar El,Boussaoudi, Ibtissam,Benkaddour, Rajae,Hamdoun, Ouafaa,Lemrini, Mounya,Martin, Patrick,Badoc, Alain,Lamarti, Ahmed The Korean Society of Plant Biotechnology 2020 식물생명공학회지 Vol.47 No.1
Here, we describe an efficient and rapid protocol for the micropropagation of Thymus pallidus Cosson ex Batt., a very rare medicinal and aromatic plant in Morocco. After seed germination, we tested the effect of different macronutrients, cytokinins alone or in combination with gibberellic acid (GA<sub>3</sub>) or auxins, on T. pallidus plantlet growth. We found that Margara macronutrients (N<sub>30</sub>K) had the best effect on the in vitro development of the plantlets. The addition of 0.93 μM/L 1,3-diphenylurea (DPU), 0.46 μM/L adenine (Ad), and 0.46 and 0.93 μM/L kinetin (Kin) resulted in the best shoot multiplication and elongation. In addition, the combination of 0.46 μM/L Kin, DPU, or Ad with gibberellic acid, in particular, 0.46 μM/L Ad + 0.58 μM/L GA<sub>3</sub> and 0.46 μM/L Kin + 1.15 μM/L GA<sub>3</sub>, led to better bud and shoot multiplication. Moreover, the integration of the combinations of 0.46 μM/L Kin and auxins, namely 0.46 μM/L Kin + 2.85 μM/L indole-3-acetic acid (IAA), 0.46 μM/L Kin + 2.85 or 5.71 μM/L indole-3-butyric acid (IBA), and 0.46 μM/L Kin + 0.3 or 0.57 μM/L 1-naphthaleneacetic acid (NAA), in the culture medium led to better root development and optimized aerial growth. Finally, the in vitro plants from the medium containing N<sub>30</sub>K + 0.46 μM/L Kin + 2.85 μM/L IAA were successfully acclimatized; these plants served as a source for repeating in vitro culture.
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Conservation of Thymus pallidus Cosson ex Batt. by shoot tip and axillary bud in vitro culture
Zineb Nejjar El Ansari,Ibtissam Boussaoudi,Rajae Benkaddour,Ouafaa Hamdoun,Mounya Lemrini,Patrick Martin,Alain Badoc,Ahmed Lamarti (사)한국식물생명공학회 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.1
Here, we describe an efficient and rapid protocol for the micropropagation of Thymus pallidus Cosson ex Batt., a very rare medicinal and aromatic plant in Morocco. After seed germination, we tested the effect of different macronutrients, cytokinins alone or in combination with gibberellic acid (GA3) or auxins, on T. pallidus plantlet growth. We found that Margara macronutrients (N30K) had the best effect on the in vitro development of the plantlets. The addition of 0.93 μM/L 1,3-diphenylurea (DPU), 0.46 μM/L adenine (Ad), and 0.46 and 0.93 μM/L kinetin (Kin) resulted in the best shoot multiplication and elongation. In addition, the combination of 0.46 μM/L Kin, DPU, or Ad with gibberellic acid, in particular, 0.46 μM/L Ad + 0.58 μM/L GA3 and 0.46 μM/L Kin + 1.15 μM/L GA3, led to better bud and shoot multiplication. Moreover, the integration of the combinations of 0.46 μM/L Kin and auxins, namely 0.46 μM/L Kin + 2.85 μM/L indole-3-acetic acid (IAA), 0.46 μM/L Kin + 2.85 or 5.71 μM/L indole-3-butyric acid (IBA), and 0.46 μM/L Kin + 0.3 or 0.57 μM/L 1-naphthaleneacetic acid (NAA), in the culture medium led to better root development and optimized aerial growth. Finally, the in vitro plants from the medium containing N30K + 0.46 μM/L Kin + 2.85 μM/L IAA were successfully acclimatized; these plants served as a source for repeating in vitro culture.
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Secondary somatic embryogenesis in Cork oak: influence of plant growth regulators
Ben Ali Naouar,Benkaddour Rajae,Rahmouni Safaâ,Boussaoudi Ibtissam,Hamdoun Ouafaa,Hassoun Mustapha,Azaroual Latifa,Badoc Alain,Martin Patrick,Lamarti Ahmed 한국산림과학회 2023 Forest Science And Technology Vol.19 No.1
Cork oak (Quercus suber L.) is one of the most important Mediterranean forest tree species, and the Ma^amora forest-Morocco is considered to be the world largest cork oak stand. Clonal propagation of Quercus suber via somatic embryogenesis is an alternative to conventional tree propagation methods. However, complete maturation of somatic embryos is considered the major bottleneck for mass propagation of Quercus suber during somatic embryogenesis. This study focuses on influences of cytokinins on secondary somatic embryogenesis. Cytokinins showed a negative effect on the induction of secondary somatic embryogenesis. For the various concentrations tested, the number of secondary somatic embryos was significantly reduced except in the case with low dose of 2iP, which showed a slight increase. Abscisic acid (ABA) may be beneficial at low concentrations between 0.3 and 0.5mg/l. The best number of secondary embryos was given at 0.5mg/l ABA with an average number of 5 embryos from each primary embryo, which represents a slight increase below 10% compared to control medium. Also, we found that the number of responsive embryos presenting secondary somatic embryos based on their cotyledons decreased once adding ABA. Phenylurea derivatives TDZ and DPU do not promoted the process of secondary somatic embryogenesis. DPU showed no significant effect with a similar response to that of the control medium and TDZ showed an inhibitory effect. Gibberellic acid (GA3) partially blocked the process of somatic embryos multiplication, but promoted remarkably the germination of cotyledonary embryos without requesting a particular protocol. Our results provide a new insight into embryo development, establishing the basis for further research toward improvement of secondary somatic embryogenesis in Moroccan cork oak.
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