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RISS 인기검색어
- 검색키워드
- 저자 : Ali Safi (검색결과 2 건)
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Mostafa Nasiri,Ali Safi 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.4
Real-time hybrid simulation evaluates the response of a structure in real time. In this study, a building with multi-story structure is divided into numerical and experimental substructures, and the vibration behavior of the experimental story is studied among the real-time simulation of the other stories. For applying the effect of static and inertial forces produced by the other stories to the experimental story, an electrohydraulic actuator and a shake table are used, respectively. The dynamics of the electrohydraulic actuator and the shake table can be estimated entirely by time delays, and these delays in the loop of simulation can reduce accuracy and cause system instability. Therefore, a delayed differential equation is used to determine the critical time delays depending on the system parameters. Results of simulation show the effect of non-dimensional parameters and time delays on the stability margin of hybrid simulation.
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Bio-hybrid dental implants prepared using stem cells with β-TCP-coated titanium and zirconia
Ihab Nabeel Safi,Basima Mohammed Ali Hussein,Ahmed Majeed Al-Shammari 대한치주과학회 2022 Journal of Periodontal & Implant Science Vol.52 No.3
Purpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells. Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with betatricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay. Implants with 3D-engineered tissue were implanted into the lower right central incisors after extraction from rabbits. Forty implants (Ti or zirconia) were subdivided according to the duration of implantation (healing period: 45 or 90 days). Each subgroup (20 implants) was subdivided into 4 groups (without cells, PDLSC sheets, BMMSC sheets, and co-culture cell sheets). All groups underwent histological testing involving haematoxylin and eosin staining and immunohistochemistry, stereoscopic analysis to measure the PDL width, and field emission scanning electron microscopy (FESEM). The natural lower central incisors were used as controls. Results: The BMMSCs co-cultured with PDLSCs generated a well-formed PDL tissue that exhibited positive periostin expression. Histological analysis showed that the implantation of coated (Ti and zirconia) dental implants without a cell sheet resulted in a well-osseointegrated implant at both healing intervals, which was confirmed with FESEM analysis and negative periostin expression. The mesenchymal tissue structured from PDLSCs only or co-cultured (BMMSCs and PDLSCs) could form a natural periodontal tissue with no significant difference between Ti and zirconia implants, consequently forming a biohybrid dental implant. Green fluorescence for periostin was clearly detected around the biohybrid implants after 45 and 90 days. FESEM showed the invasion of PDL-like fibres perpendicular to the cementum of the bio-hybrid implants. Conclusions: β-TCP-coated (Ti and zirconia) implants generated periodontal tissue and formed biohybrid implants when mesenchymal-tissue-layered cell sheets were isolated from PDLSCs alone or co-cultured BMMSCs and PDLSCs.
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