http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Akram M. El Habab (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Hoda E. Mohamed,Mervat E. Asker,Nahla S. Kotb,Akram M. El Habab 대한혈액학회 2020 Blood Research Vol.55 No.1
BackgroundFetal bovine serum (FBS) has been used to support the growth and proliferation of mam-malian cells for decades. Owing to several risk factors associated with FBS, several trials have been conducted to evaluate substitutes to FBS with the same efficiency and the lower risk issues.MethodsIn this study, human platelet lysate (HPL) derived from activated human platelets was evaluated as an alternative to FBS due to the associated risk factors. To evaluate the effi-ciency of the preparation process, platelet count was performed before and after activation. The concentrations of several growth factors and proteins were measured to investigate HPL efficiency. HPL stability was studied at regular intervals, and optimal hep-arin concentration required to prevent gel formation in various media was determined. The biological activity of HPL and FBS was compared by evaluating the growth perform-ance of Vero and Hep-2 cell lines.ResultsResult of platelet count assay revealed the efficiency of HPL preparation process. Growth factor concentrations in HPL were significantly higher than those in FBS, while the protein content of HPL was lower than that of FBS. Stability study data showed that the prepared HPL was stable for up to 15 months at -20oC. Ideal heparin concentration to be used in different media was dependent on calcium concentration. Results of cell viability assay showed that HPL was superior to FBS in supporting the growth and proliferation of Vero and Hep-2 cells. ConclusionThe HPL prepared by the mechanical activation of platelets may serve as an efficient alter-native to FBS in cell culture process.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
