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      • Automatic Multi-Operation Machine Using Low Cost Color Sensor and Plc

        D. Ajay abilash,P. Kayalvizhi,C. Ramachandra Durai 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2

        Sensors play a major role in an automated manufacturing system. They are used for getting information from manufacturing operations and processes that are being performed. They are also used for making decisions about the operations being conducted. The purpose of sensors is to inspect and monitor the work-in-progress interface with the manufacturing equipment. In manufacturing industries the multi-operation machine like VMC (Vertical machining center) and HMC (Horizontal machining center) are the major part. It is used to improve the high productivity, high quality and high accuracy of the product. But the CNC machine has some drawbacks like high initial investment, high maintenance and skilled labor wants to operate the machine. To overcome these problems, this paper proposes a low cost automatic multi-operation machine. It performs basic machining operations like drilling, tapping and boring using low cost color sensors (LDR and LEDs). In this system color sensors are the main part to determine the machining operations based on the usage of color LEDs. If LDR detects the color LED the machining operation will take places, the machining operation and job clamping operation are achieved by pneumatic system. All these operations are carried out in a sequence by pre-programming the Programmable Logic Controller.

      • Automation in Biomethanation Plant Using PLC and SCADA

        D. Ajay abilash,P. Kayalvizhi,R. Rakesh,S. Balamurugan 보안공학연구지원센터 2016 International Journal of Bio-Science and Bio-Techn Vol.8 No.2

        Biomethanation is a feasible method to generate the biogas from organic waste like food waste, human waste, night soil waste, cow dung etc. The night soil waste and animal waste has bad odor in nature and it produces some infection to operators, so we implement the automation in biomethanation plant. This plant has four major processes like waste collection and mixing, digestion, gas collection and gas distribution. These above processes are controlled by manually, hence it produces some problems and errors, to overcome this problem, and we proposed an automatic control system for biomethanation plant. In this system the overall process are controlled by programmable logic controller and field instruments. And also these processes are controlled and monitored by SCADA using wonderware intouch software.

      • Clinical, Cytogenetic and CYP1A1 exon-1 Gene Mutation Analysis of Beedi Workers in Vellore Region, Tamil Nadu

        Sundaramoorthy, Rajiv,Srinivasan, Vasanth,Gujar, Jidnyasa,Sen, Ayantika,Sekar, Nishu,Abilash, Valsala Gopalakrishnan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

        Background: Beedi rollers are exposed to unburnt tobacco dust through cutaneous and pharyngeal route and it is extremely harmful to the body since it is carcinogenic in nature and can cause cancer during long exposure. This indicates that occupational exposure to tobacco imposes considerable genotoxicity among beedi workers. Materials and Methods: In the present study, 27 beedi workers and age and sex matched controls were enrolled for clinical, cytogenetics and molecular analysis. Clinical features were recorded. The workers were in the age group of 28-67 years and were workers exposure from 8-60 years. Blood samples were collected from workers and control subjects and lymphocyte cultures were carried out by using standard technique, slides were prepared and 50 metaphases were scored for each sample to find the chromosomal abnormalities. For molecular analysis the genomic DNA was extracted from peripheral blood, to screen the variations in gene, the exon 1 of CYP1A1 gene was amplified by polymerase chain reaction (PCR) and then screened with Single Strand Conformation Polymorphism (SSCP) analysis. Results: A statistically significant increase was observed in the frequencies of chromosomal aberrations in exposed groups when compared to the respective controls and variations observed in Exon 1 of CYP1A1(Cytochrome P450, family 1, subfamily A, polypeptide 1) gene. Conclusions: This study shows that, the toxicants present in the beedi that enter into human body causes disturbance to normal state and behavior of the chromosomes which results in reshuffling of hereditary material causing chromosomal aberrations and genomic variations.

      • Multi-Parameter Approach for Evaluation of Genomic Instability in the Polycystic Ovary Syndrome

        Sekar, Nishu,Nair, Manju,Francis, Glory,Kongath, Parvathy Raj,Babu, Sandhya,Raja, Sudhakaran,Gopalakrishnan, Abilash Valsala Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

        Background: The polycystic ovary syndrome (PCOS), characterized by hyperandrogenism and chronic anovulation, is a common endocrine disorder in women. PCOS, which is associated with polycystic ovaries, hirsutism, obesity and insulin resistance, is a leading cause of female infertility. In this condition there is an imbalance in female sex hormones. All the sequelae symptoms of PCOS gradually lead to cancer in the course of time. It is heterogeneous disorder of unknown etiology so it is essential to find the exact cause. Materials and Methods: In this study both invasive and non-invasive techniques were employed to establish the etiology. Diagnosis was based on Rotterdam criteria (hyperandrogenism, ovulatory dysfunction, PCOM) and multiparameters using buccal samples and dermatoglypic analysis and cytogenetic study for 10 cases and four age and sex matched controls. Results: In clinical analysis we have observed the mean value of total testosterone level was 23.6nmol/L, total hirsutism score was from 12-24, facial acne was found in in 70% patients with 7-12 subcapsular follicular cysts, each measuring 2-8 mm in diameter. In dermatoglypic analysis we observed increases in mean value ($45.9^{\circ}$) of ATD angle when compared with control group and also found increased frequency (38%) of Ulnar loops on both fingers (UU), (18%) whorls on the right finger and Ulnar loop on left finger (WU) and (16%) arches on right and left fingers (AA) were observed in PCOS patients when compared with control subjects. Features which could be applied as markers for PCOS patients are the presence of Ulnar loops in middle and little fingers of right and left hand. The buccal micronucleus cytome assay in exfoliated buccal cells, we found decrease in frequency of micronuclei and significant increases in frequency of karyolysed nuclei in polycystic ovarian syndrome patients. Chromosome aberration analysis revealed a significant increase in frequency of chromosome aberrations (CAs) in PCOS patients when compared with controls. Conclusions: From this present work it can be concluded that non-invasive technique like dermatoglypics analysis and buccal micronucleus cytome assays with exfoliated buccal cell can also be effective biomarkers for PCOS, along with increased CAs in lymphocytes as a sign of genetic instability. There is a hypothesis that micronuclei and chromosomal aberrations could have a predictive value for cancer. From this present work it can be concluded to some extent that non-invasive technique like dermatoglypics and buccal cell analysis can also be effective for diagnosis.

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