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        Genetic Diversity and Differentiation of Colletotrichum spp. Isolates Associated with Leguminosae Using Multigene Loci, RAPD and ISSR

        Mahmodi, Farshid,Kadir, J.B.,Puteh, A.,Pourdad, S.S.,Nasehi, A.,Soleimani, N. The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.1

        Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of multigene sequences, the Colletotrichum species showed diverse degrees of intera and interspecific divergence (0.0 to 1.4%) and (15.5-19.9), respectively. A multilocus molecular phylogenetic analysis clustered Colletotrichum spp. isolates into 3 well-defined clades, representing three distinct species; C. truncatum, C. dematium and C. gloeosporioides. The ISSR and RAPD and cluster analysis exhibited a high degree of variability among different isolates and permitted the grouping of isolates of Colletotrichum spp. into three distinct clusters. Distinct populations of Colletotrichum spp. isolates were genetically in accordance with host specificity and inconsistent with geographical origins. The large population of C. truncatum showed greater amounts of genetic diversity than smaller populations of C. dematium and C. gloeosporioides species. Results of ISSR and RAPD markers were congruent, but the effective maker ratio and the number of private alleles were greater in ISSR markers.

      • KCI등재

        Genetic Diversity and Differentiation of Colletotrichum spp. Isolates Associated with Leguminosae Using Multigene Loci, RAPD and ISSR

        Farshid Mahmodi,J. B. Kadir,A. Puteh,A. Nasehi,N. Soleimani,S. S. Pourdad 한국식물병리학회 2014 Plant Pathology Journal Vol.30 No.1

        Genetic diversity and differentiation of 50 Colletotrichumspp. isolates from legume crops studied through multigeneloci, RAPD and ISSR analysis. DNA sequence comparisonsby six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3)verified species identity of C. truncatum, C. dematium andC. gloeosporiodes and identity C. capsici as a synonymof C. truncatum. Based on the matrix distance analysis ofmultigene sequences, the Colletotrichum species showeddiverse degrees of intera and interspecific divergence (0.0 to1.4%) and (15.5-19.9), respectively. A multilocus molecularphylogenetic analysis clustered Colletotrichum spp. isolatesinto 3 well-defined clades, representing three distinctspecies; C. truncatum, C. dematium and C. gloeosporioides. The ISSR and RAPD and cluster analysis exhibited a highdegree of variability among different isolates and permittedthe grouping of isolates of Colletotrichum spp. into threedistinct clusters. Distinct populations of Colletotrichum spp. isolates were genetically in accordance with host specificityand inconsistent with geographical origins. The largepopulation of C. truncatum showed greater amounts ofgenetic diversity than smaller populations of C. dematiumand C. gloeosporioides species. Results of ISSR and RAPDmarkers were congruent, but the effective maker ratio andthe number of private alleles were greater in ISSR markers.

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