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유방암 유즙에서 분리한 포도구균의 분자생물학적 typing과 multiplex PCR을 이용한 장독소의 검출
김신 ( Shin Kim ),홍현표 ( Hyon Pyo Hong ),김상윤 ( Sang Yun Kim ),권헌일 ( Heon Il Kwon ),이희무 ( Hee Moo Lee ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3
Forty strains of Staphylococcus aureus were isolated from mastitic milk. As a result of antimicrobial susceptibility test, the strains of S aureus revealed 47.5% were resistant to ampicillin and penicillin, and 7.5% to gentamicin. But 45% of isolates were sensitive to antimicrobial agents tested. In case of enterotoxin production, 56.3% of 16 strains produced enterotoxin D. Two strain of enterotoxin D producers produced both enterotoxin B and D. According to isolation date, 15 representative strains were selected. As a results of pulsed field gel eletrophoresis analysis of the 15 representative strains, 14 strains were identical. Therefore we consider the identical strains of S aureus have caused continuously bovine mastitis in this dairy farm. If autogenous vaccine can be made by the strains, it will work well for the prevention of bovine mastitis caused by S aureus.
경북지역 가축에서 분리된 Salmonella typhimurium과 S enteritidis의 phage typing 및 pulsed-field gel electrophoresis
김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.3
Forty-five Salmonella typhimurium isolates were encountered 8 phage types in which DT197 and U302 were the predominant types. The DT104 type which was first found from pig in Korea, and was resistant to chloramphenicol, streptomycin, sulfamethoxazole/trimethoprim, tetracycline, gentamicin and nalidixic acid. Twenty-two S enteritidis isolates were encountered 5 phage types in which PT4 were the representative (predominant). S enteritidis isolates were susceptible to all antimicrobial agents. As a result of PFGE analysis for S typhimurium and 5 enteritidis, PFGE patterns was better than phage typing in discriminating of strains. PFGE patterns were not in accord with phage type even though some strain had the same phage types.
경북지역 가축에서 분리된 Salmonella typhimurium과 S enteritidis의 병원성 시험
김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
The result of studying the pathogenicity of Salmonella typhimuriwn and S enteritidis isolated from domestic animals in Gyeongbuk province were summarized as follows. In Congo-red binding test, S typhimurium had much more rough types than S enteritidis. In colicin production test, 4 strains of S typhimurium were positive but all of S enteritidis were negative. In hemolysin production test, all of S typhimurium and S enteritidis were negative. In Guinea pig serum resistant test, all of S typhimurium and S enteritidis were positive. As a result of pathogenicity test to mice, 54.4% of mice were died. Therefore, S typhimurium and S enteritidis were considered as highly pathogenic. S typhimurium DT104 and S enteritidis PT4 were more pathogenic to mice than other phage types of same serovar. S typhimurium and S enteritidis were considered not so pathogenic for 6-day-old chickens. The recovery rates of Salmonella strains from mice and chickens inoculated were 96.8%, and 54%, respectively. In chickens, proportional to the time From 2 weeks after challenge inoculation. The recovery rates were noticeably decreased.
경북지역 가축에서 Salmonella속 균 감염증에 대한 역학적 특성
김상윤 ( Sang Yun Kim ),이희무 ( Hee Moo Lee ),김신 ( Sin Kim ),홍현표 ( Hyon Pyo Hong ),권헌일 ( Heon Il Kwon ) 한국가축위생학회 2001 韓國家畜衛生學會誌 Vol.24 No.1
The result of studying the epidemiological characteristics of Salmonella strains which have been isolated from the domestic animals in Gyeongbuk province from February 1998 to August 2000 were summarized as follows. The isolation rates of Salmonella strains were 2.0% from cattle feces, 6.3% from cattle lymph node, 9.5% from pig feces, and 25.1% from pig lymph node. In poultry, the isolation rates were 30.3%. The isolates of Salmonella showed positive reaction for MUCAP test, methyl red test, but showed negative reaction for urea test, indole test, Voges Proskauer test. On TSI agar, the isolates showed acid butt, alkaline slant. Also, the isolates were identified as Salmonella strain by API 20E kit. Non H2S production Salmonella strains isolated from poultry were identified as S gallinarum. As a result of serotyping, B group were the most common in cattle and pig, D1 in chickens. 21 serovars were found. The common serovar from the domestic animals was S typhimurium, S derby, S agona, S schwarzenground, S enteritidis and S gallinarum. The most commonly encountered serovars in cattle were S agona and S typhimurium in pig, S gallinarum in chicken. As a result of antimicrobial susceptibility test, all Salmonella isolates were susceptible to amikacin, ciprofloxacin. Norfloxacin, cefotaxime and polymycin B. The resistance rates to tetracycline and streptomycin was 58% and 56% respectively. 69.3% of all isolates were resistant to more than one antimicrobial agent. Out of the resistant isolates, the isolates resistant to streptomycin and tetracycline was 36%. There were 24 strains of multiresistant isolates resistant to more than 5 antimicrobial agents. S typhimurium were resistant to all antimicrobial agents, also had a lot of multiresistant strains. Therefore, S typhimurium was considered as a major agent of antimicrobial resistance.
경북지역 애견 번식장에서 분리한 Brucella canis의 생화학적특성 및 PFGE 양상
김성국 ( Seong Guk Kim ),김영환 ( Young Hoan Kim ),홍현표 ( Hyon Pyo Hong ),엄현정 ( Hyun Jung Eom ),장성준 ( Seong Jun Jang ),조민희 ( Min Hee Jo ),이양수 ( Yang Soo Lee ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.3
A biochemical characterization and antimicrobial drugs susceptibility study was conducted in four breeding kennel which was canine abortion caused by Brucella canis in Gyeongbuk province in 2003-2006. Total of 267 dogs domesticated in the four kennel were examination. Among them, 143(53.6%) dogs were sero-positive and 25 of blood samples were isolated to Brucella canis. At amplification of 35KDa-BCSP gene using PCR, 711bp DNA fragment was same visible in 25 isolates and B canis RM6/66. Biochemical characterization of B canis isolated was non-hemolytic, no production of H2S, no fermentation of carbohydrates, catalase-positive, oxidase-positive, indol-negative, hydrolyzation of urea, reduction of nitrate and development of thionin dye medium. Using disk-diffusion method, all of 25 strains tested were found to be highly susceptible to tetracycline, aminoglycoside, quinolone, macrolide antibiotics, rifampin and ampicillin in vitro. Using PFGE with restriction enzyme SmiⅠ, 25 isolates tested were typed to 2 pattern, S1 and S2.