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WonJin Kim(김원진),JiUn Lee(이지운),Gi-Hoon Yang(양지훈),Hyeongjin Lee(이형진),YongBok Kim(김용복),Minseong Kim(김민성),YoungWon Koo(구영원),GeunHyung Kim(김근형) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
Collagen- based cell-printing technology has provided a new strategy for tissue engineering. However, although collagen-based scaffolds can provide an outstanding biofunctional benefits, their low mechanical strength and poor controllability have been limitation for their usage as hard tissue regeneration. To overcome this limitation, α-tricalcium phosphate (α-TCP) has been used for biomedical scaffolds. α-TCP is biocompatible and soluble material, and hydrolyzed rapidly to calcium-deficient hydroxyapatite which makes α-TCP a useful material for bone tissue regeneration. in this study, we fabricate 3-dimensional (3D) scaffold based on α-TCP/collagen struts coated with collagen-based bioink. To compare the physical and cellular activities, we used a scaffold composed of α-TCP/collagen scaffold coated with cell-embedded collagen. Following fabrication of the cell (MC3T3-E1)-embedded a-TCP/collagen scaffold, the cellular activities were evaluated in vitro.
저온 프린팅 공정을 통한 콜라겐/실크-피브로인/세포외기질이 포함된 조직재생용 세포담체 개발
허준태(JunTae Huh),이형진(Hyeongjin Lee),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),구영원(YoungWon Koo),여미지(Miji Yeo),임정형(JungHyung Lim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In tissue engineering, biomaterials must be biocompatible, biodegradable, and mechanically stable to be used for biomedical scaffold. Among various biomaterials, natural polymers are widely used for great biocompatibility. However, due to their low mechanical properties, it has been a challenge to build desired 2D/3D structure using natural polymers. To overcome this problem, in this study, we used low temperature 3D printing to create a scaffold with collagen/silk-fibroin(SF)/extracellular matrix(ECM). Then, fibroblast cells were grown on the fabricated scaffold. Various in vitro cellular activities (cell-viability, MTT assay) for pure collagen, pure SF, collagen/SF, and collagen/SF/ECM scaffolds were compared. The collagen/SF/ECM scaffold showed 30% higher cell metabolic activities than pure collagen scaffold.
골 조직 재생을 위한 전기수력학적 공정을 이용한 섬유구조의 PCL/ceramic 세포담체 제작
김민성(Minseong Kim),이형진(Hyeongjin Lee),전호준(HoJun Jeon),여명구(MyungGu Yeo),김용복(YongBok Kim),양지훈(Gi-Hoon Yang),김원진(WonJin Kim),김근형(GeunHyung Kim) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12
In this study, we fabricated a new ceramic fibrous scaffold, using the initial jet of an electrospinning process and ethanol media as a target. The fabricated three-dimensional (3D) fibrous ceramic structure was configured with multilayered micro-sized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes (ECMs). The fabrication of the ceramic structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous ceramic scaffold was cultured with pre-osteoblasts to observe the initial cellular activities in comparison with a solid freeform fabricated 3D scaffold sharing a similar structural geometry. The micro/nanofibrous 3D fibrous ceramic strut scaffold exhibited significantly high initial cell attachment, proliferation, and viability compared to solid freeform fabricated 3D scaffold.