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아카바네 생바이러스 백신 접종에 의한 한우 백혈구 아군의 변화
박영남 ( Young Nam Park ),손승미 ( Sung Mi Son ),신명균 ( Myung Kyun Shin ),권명상 ( Myung Sang Kwon ) 한국가축위생학회 2006 韓國家畜衛生學會誌 Vol.29 No.3
To identify immune response of leukocytes in peripheral blood of cattle vaccinated with an attenuated live Akabane virus vaccine, leukocytes were reacted with monoclo-nal antibodies which are specific to bovine lymphocyte surface antigens and assayed by the flow cytometry. Serum neutralizing(SN) test was used to measure antibody titers after vaccination. SN antibody was appeared to 7 days post-vaccination(PV) and 2-8 antibody titers were observed in 14 days PV. Proportion of CD8-MHCclassⅡ+ expressing cells were rapidly increased at 3 days PV. CD8+MHCclassⅡ- cells were increased at 7 days PV. CD4+CD8-, WC+CD4-, CD4+CD8+, WC1-CD4+, WC1-CD8+, and CD4-CD8+ cells were highly increased at 3, 3, 7, 7, 14, 14 days PV, respectively.