http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
마이크로파 추출법을 이용한 Negella sativa중의 휘발물질 분리: 추출물이개와 쥐의 CYP1A에 미치는 영향
박종혁 ( Jong Hyouk Park ),유학 ( Xue Liu ),김성우 ( Sung Woo Kim ),심재한 ( Jae Han Shim ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-
The volatile components of Nigella sativa seeds were isolated using microwave-assisted extraction (MAE) and identified using gas chromatography. Further investigations were carried out to demonstrate the effects of wholeextracts on canine (dog) and murine (rat) cytochrome P450 1A (CYP1A). The optimal extraction conditions of MAE wereas follows: 25 mL of water, medium level of microwave oven power and 10 min of extraction time. A total of 32 compoundswere identified under the conditions using GC-FID and GC-MS. Thymoquinone (38.23%), p-cymene (28.61%), 4-isopropyl-9-methoxy-1-methyl-1-cyclohexene (5.74%), longifolene (5.33%), a-thujene (3.88) and carvacol (2.31%) werethe main compounds emitted from N. sativa seeds. Various extracts including pure compounds, essential oil, nonpolarpartition, relatively high-polar/nonpolar partition, and polar partition extracts effectively inhibited the reaction ofethoxyresorufin O-de-ethylation, which is specified for CYP1A activity both in dog and rat. This in vitro data shouldbe heeded as a signal of possible in vivo interactions. The use of human liver preparations would considerablystrengthen the practical impact of the data generated from this study.