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유전자조작, 균주분리 간장 유래 혈전분해 효소 생산 균주의 분리 및 배양학적 특성
백성열 ( Seong Yeol Back ),윤혜주 ( Hye Ju Yun ),박희동 ( Heui Dong Park ),여수환 ( Soo Hwan Yeo ) 한국미생물생명공학회 2011 한국미생물·생명공학회지 Vol.39 No.4
Bacterial strains exhibiting fibrinolytic activity were screened from traditional Korean soybean sauce. The Fibrinolytic activities of the various isolated microorganism were further examined and the superior strain YJ11-21 was selected for further analyses. Gene sequence analysis of 16S rDNA of the YJ11-21 strain revealed Bacillus lichenformis. Optimal culture conditions were investigated in order to maximize the production of the fibrinolytic enzyme by YJ11-21. Amongst the carbon sources tested, glucose was the most effective for enzyme production and amongst the nitrogen sources tested, yeast extract was seen to be the most effective. A one percent addition of NaCI to the medium resulted in the highest fibrinolytic activity. Interestingly, a 10% addition of NaCI resulted in a high activity together with a high cell growth rate. Therefore, YJ11-21 is speculated of being a halotolerant. The optimum pH and temperature for enzyme production were a pH of 9.0 and 30℃, respectively.