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黃周光,라파포트.엘 충북대학교 농업과학기술연구소 1992 農業科學硏究 Vol.10 No.1
Callus from tissue- cultured petioles of celery (Apium graveolens var. dulce) plants on modified MS medium were employed for cell suspension culture. When sufficient number of cells were obtained, they were pipetted to regeneration medium. The presence of auxin prevented regeneration, but addition of zeatin made no difference in the number of plants regenerated. Highest number of plants regenerated was obtained when cell clusters were used as inoculum to the regeneration medium. One of the objectives of present study is to observe DNA base changes induced through tissue culture by recently developed RAPD method. However, all 12 primers used in this experiment foiled to detect genetic variation in regenerated celery plants. Further study with more primers under better environmental control is proposed.