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Lee, Cheol-Ho,Luo, Yao-Wu 진주산업대학교 1997 論文集 Vol.36 No.-
晉州産業大學校와 中國河北農業大學과의 飼料用 수수 品種開發에 관한 共同硏究協約을 締結하고 同質四倍體植物의 表現型上特徵이 營養器官의 巨大化와 成分含量의 增加를 誘發하는 특성을 이용하여 한국과 중국에서 수집된 二倍體수수에 콜히친을 처리하여 四倍體수수를 만들고 四倍體수수와 이들의 雜種을 대상으로 主要形質의 유전적 특성과 세포분열시 염색체분리현상을 규명하여 사료용 품종 개발에 필요한 수수육종의 기초자료로 활용하고져 하였으며 그 결과를 요약하면 다음과 같다. 1. F_1을 自殖하여 얻은 同質四倍體수수(2n=40)는 二倍體수수에 비해 種實이 현저히 크고 穀實收量이 많았으며 蛋白質과 아미노산含量도 높았다. 2. 同質四倍體수수의 杆長과 穗長은 二倍體수수와 큰 차이를 보이지 않았으나 着粒數는 同質四倍體수수의 초기세대에서 적었고, 二倍體와 四倍體수수를 교잡한 三倍體雜種의 着粒數는 親系 四倍體수수보다 많았다. 3. 同質四倍體에 있어서 四價染色體와 減數分列 後期의 1, 2개 染色體 缺失 및 4개의 生殖細胞의 발생빈도는 매우 다양했으며, 四價染色體의 頻度가 結實率에 중요한 인자로서 작용하지 않았고 染色體 缺失의 頻度와도 有意的인 관련이 없었다. 4. 異數體(2n=39, 41)는 倍數體(2n=40)보다 染色體 缺失率이 높고 정상적인 생식세포의 빈도가 낮았다. 5. 受粉樣式에 따른 結實率은 四倍體 系統間에 차이가 많았고 着粒數가 많은 系統의 選拔과 잡종생산을 위한 이들 계통의 이용 가능성을 시사하였다. The forage traits and chromosome behavior or autotetraploid sorghum (Sorghum bicolor Moench) inbreds and F_1 hybrids were investigated. Autotetraploid sorghum inbreds(2n=40) had heavier kernel weight, more seed yield, and larger protein and amino acid content than their diploid counterparts. The stalk height and panicle length of the autotetraploids, however, were not different from those of the diploids, and the seed set of autotetraploids was lower in the early generations. The seed set of tetraploid F_1 hybrids was related to and higher than that of the tetraploid parental inbred lines. The frequency of quadrivalents, anaphase Ⅰ and Ⅱ laggards, and quartet micronuclei varied among autotetraploids. The frequency of quadrivalents did not appear to be an important factor in seed set, nor was it significantly associated with the frequency of laggards. Aneuploids(2n=39,41) had higher percentage of laggards and micronuclei than euploids(2n=40). Methods of pollination significantly affected the seed set in the tetraploid S_4 lines. Differences in seed set among autotetraploid lines indicated the feasibility of selecting the lines with higher seed set and utilizing those lines for hybrid production.
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李喆浩,羅耀武,李宗玉,常金華 진주산업대학교 농업기술연구소 1998 農業技術硏究所報 Vol.11 No.-
사료용 수수의 육종을 위한 기초자료를 얻기 위하여 1996년부터 1997년까지 국내에서 수집할 수 있는 재래종을 수집하여 사료가치가 인정되는 10개 형질들의 성적을 農村振興廳作物試驗場의 수수조사기준에 의해 조사하여 사료용으로 적합한 地上部生體重과 株富種實重을 중심으로 유전력은 Robinson et al, Grafius et al의 廣義의 유전력을, 表現型相關, 遺傳相關 및 環境相關은 Robinson et al의 방법을, 經路係數는 Wright, Dewey and Lu의 방법을 적용, 분석하였던 바 그 결과를 요약하고져 한다. 1. 遺傳力은 葉幅, 穗長 및 千粒重이 높았고, 地上部生體重, 草長, 株當種實重은 중간정도였다. 2. 형질간의 相關分析에서 地上部生體重은 株當種實重, 千粒重, 株當葉數 및 葉幅과 높은 正相關이 인정되었고 株當種實重은 千粒重, 葉幅, 穗當着粒數 및 開花日數와 높은 正의 相關을 나타냈으며 그 외 형질간의 상관에서는 葉幅이 대부분의 형질과 높은 상관을 나타냈다. 3. 株當種實重에 미치는 직접효과는 千粒重이 가장 높았고 穗當着粒穗, 地上部生體重의 順이었으며, 葉幅은 가장 낮은 직접효과를 나타냈고, 地上部 生體重에 미치는 직접효과는 葉幅과 草長이 가장 높았고 그외의 형질은 이들 형질에 영향을 주므로서 수량에 공헌하는 것으로 나타났다. 이러한 결과로 미루어 사료용 수수의 선발대상형질은 株當種實株을 목표로 할 경우 開花日數가 길고, 葉幅이 넓고, 穗當着粒數가 많으며 大粒種을 선발함이 유리하며, 생초용 등을 목표로 할 경우에는 葉幅이 넓고 키가 큰 것이 유리하며, 다른 형질들은 이들 두형질에 영향을 미치므로서 수량에 영향을 미치는 것으로 분석되었다. This experiment was conducted to estimate genetic parameters such as heritabilities, and phenotypic, genotypic and evironmentals correlations for some agronomic characters, and the path-coefficient of each character influencing the grain yield and top fresh weight in selected domestic varieties of sorghum(Sorghum bicolor Moench) in Korea. The different sorghum lines were planted in a randomized block design with three replications, and were measured for 10 quantitative characters, i.e., days to flowering, plant height, stem diameter, leaf width, number of leaves per plant, fresh weight of top part per plant(yield 1), panicle length, number of grain per panicle, 1,000 grain weight and grain weight per plant(yield 2). The high heritability values were estimated for leaf width(0.9578), panicle length(0.9468) and 1,000 grain weight(0.9140), but number of leaves per plant, number of grain per panicle and grain weight per plant were lower than the other characters. The phenotypic correlation coefficients among all pairs of characters were slightly lower than the corresponding genotypic correlations, and fresh weight of top part per plant(yield 1) was highly positively correlated with grain weight per plant, 1,000 grain weight, number of leaves per plant and leaf width, and grain weight per plant(yield 2) highly positively correlated with 1,000 grain weight, leaf width and days to flowering and it could be concluded that 1,000 grain weight, leaf width and grain weight per plant, are the important characters in sorghum breeding. In the path-coefficient of each character influencing the fresh weight of top part per plant(yield 1), two characters which are leaf width and plant height had the higher direct effects upon fresh weight yield respectively, and in the path-coefficient of each character influencing of grain weight per plant(yield 2), three characters which are 1,000 grain weight, number of grain per panicle and fresh weight of top part per plant had the higher direct effects upon seed yield, and the other characters showed higher indirect effects influencing 1,000 grain weight, number of grain per panicle and fresh weight of top part per plant. In the case of sorghum breeding for obtain the higher fresh weight, two characters which are leaf width and plant height were related very tightly for yield, otherwise, in the case of obtain the higher grain weight, three characters which are 1,000 grain weight, number of grain per panicle and fresh weight of top part per plant were related very closely. For these reasons, it was realized that the parameter for selection should be obtained on the basis of the data from four agronomic characters, i.e., leaf width, plant height, 1,000 grain weight and number of grain per panicle.
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李喆浩,李宗玉,羅耀武 진주산업대학교 농업기술연구소 2000 農業技術硏究所報 Vol.13 No.-
Interspecific hybrid between autotetraploid Sorghum(S. bicolor(L.) Moench) and Johnsongrass (S. halepense(L.) Pers) were produced using hand and plasticbags emasculation. The objectives of this research were to study cytogenetics and POD spectrum of the hybrids. The forage value of interspecific hybrid was also performed. The results showed as follows; 1. The average chromosome configuration per cell at meiotic M I of F_1 hybrid's PCMs was 0.17 Ⅰ+14.11Ⅱ+0.028Ⅲ+2.87Ⅳ+0.004Ⅴ+0.007Ⅵ+0.0007Ⅶ. At anaphase Ⅱ, unsynchronized distribution was observed expect laggards, it was negatively and significantly correlated with the number of chain quadrivalents of parent sorghum (r=-0.8718). 2. The POD spectrum of interspecific hybrid was tendency to that of Johnsongrass, and exhibited characteristic bands of Johnsongrass. Index of spectrum similarity between F_1 and parent Sorghum, Johnsongrass were 58.00%, 68.69%, respectively. The genomic model of Johnsongrass and the way of transferring desirable genes to cultivated sorghum from Johnsongrass were also discussed.
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