http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
SYMPOSIA / S13-5 : TRANSCRIPTION REGULATION BY HISTONE ACETYLASES
(D . Steger),(A . Hassan),(K . Neeley),(M . Vignali),(A . Wallberg),(R . Utley),(K . Ikeda),(S . John),(A . Eberharter),(P . Grant),(M . Pray Grant),(A . Wright),(J . Warkman) 한국생화학분자생물학회 (구 한국생화학회) 1999 6th IUBMB SEOUL CONFERENCE Vol.- No.-
New Antisense RNA Systems Targeted Against Plant Pathogens
Matousek, J.,Vrba, L.,Kuchar, M.,Pavingerova, D.,Orctova, L.,Ptacek, J.,Schubert, J.,Steger, G.,Beier, H.,Riesner, D. The Korean Society of Plant Biotechnology 2000 식물생명공학회지 Vol.27 No.5
tRNA and 7SL RNA based antisense vehicles were prepared by inserting conserved anti-viral and anti-viroid domains. Anti-PVS coat protein leader sequence (ACPL) and antistructural antihairpin domain of PSTVd (AHII) were inserted in tRNA cassette; anti- zing finger domain of PVS, AHII and anti hop latent viroid ribozyme were inserted in 7SL RNA gene isolated from A. thaliana. These constructs were shown to be transcribed both, in in vitro and in in vivo conditions. However, it followed from our work that closely linked position of PoIII reference genes and PoIIII antisense genes within T-DNA lead to the impairment of RNA expression in transgenic plants. To assay in vivo transcription of antisense genes, hairy root potato cultures were established using h. tumefaciens A4-24 bearing both, Ri plasmid and PoIII-promoterless plant expression vectors with antisense RNA genes. Expression of antisense RNA in transgenic potato tissues was proven by specific RT-PCR reactions.