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( Peng Lii ),( Ke Ping Chen ),( Hua Jun Yang ),( Qin Yao ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.15 No.2
As the genome of B.mori is available in GenBank and the EST database of B.mori is expanding, identification of novel genes of B.mori is conceivable by data-mining techniques. We used the in silico cloning method to get the vacuolar-type H(+)-ATP synthetase (V-ATPase) c subunit (16 kDa proteolipid subunit) gene of B.mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and sequencing. The V-ATPase c subunit cDNA contains a 468bp ORF. The ORF encoded a 155-residue protein that showed extensive homology with V-ATPase c subunits from other 15 species and contained four membrane-spanning helices. Tissue expression pattern analysis revealed that V-ATPase c expressed strongly in Mal-pighian tubules, not in fat body. This gene has been registered in GenBank under the accession number EU082222.