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RISS 인기검색어
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- 저자 : ( Ming Shou Lu ) (검색결과 4 건)
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( Ming Shou Lu ),( Soo Jin Lee ),( Bo Rim Kim ),( Chang Hun Park ),( Min Kyu Oh ),( Kyung Moon Park ),( Sang Yup Lee ),( Jin Won Lee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.5
2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/ Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.
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Water spray heat transfer through a piezoelectric atomizer with a single-hole micronozzle
Shou-Shing Hsieh,Ching-Feng Huang,Yung-Ming Lu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.8
We report an experimental study on the flow and heat transfer for a single microhole of water spray impingement on an indium tin oxide (ITO) heating plate using a piezoelectric atomizer. A microhole of d j = 35 µm was used and tested with a volumetric flow rate of 0.22 cm 3 /min for three different spray heights of 10, 20 and 30 mm and five heater initial temperatures of 25 o C, 50 o C, 100 o C, 150 o C, and 200 o C. Through the optical measuring techniques of the microparticle image velocimetry (µPIV) as well as interferometric particle imaging (IPI) and micro laser-induced fluorescence (µLIF), the velocity field, such as spray centerline velocity, droplet impact velocity and impact crater diameter, including impinged liquid film thickness and heat transfer performance (CHF) can be measured and calculated. The effects of the spray height and initial heater temperature on the flow and thermal characteristics are presented and discussed herein. The experimental results show that both the spray centerline velocity and spray droplet impact velocity were significantly influenced by the initial surface temperature as well as by the spray height. As a result, the cooling performance would be, in turn, affected by the aforesaid two parameters.
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Dynamic Modeling of Lactic Acid Fermentation Metabolism with Lactococcus Lactis
Euh Lim Oh,Ming Shou Lu,Chang Hun Park,Han Bin Oh,Sang Yup Lee,Jin Won Lee 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.2
A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).
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( Kim Bo Rim ),( Soo Jin Lee ),( Joo Hong Park ),( Ming Shou Lu ),( Min Kyu Oh ),( Young Rok Kim ),( Jin Won Lee ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9
2, 3-Butanediol (2, 3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2, 3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2, 3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene to generate plasmids pSB1-7. The plasmids were then introduced into K. pneumoniae using electroporation. All strains were incubated in flask experiments and 2, 3-BD production was increased by 60% in recombinant bacteria harboring pSB04 (budA and budB genes), compared with the parental strain K. pneumoniae KCTC2242. The maximum 2, 3-BD production level achieved through fedbatch fermentation with K. pneumoniae SGJSB04 was 101.53 g/l over 40 h with a productivity of 2.54 g/l.h. These results suggest that overexpression of 2, 3-BD synthesisrelated genes can enhance 2,3-BD production in K. pneumoniae by fermentation.
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