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Oxidative Potential of Some Endophytic Fungi Using 1-Indanone as a Substrate
( Fill Taicia Pacheco ),( Jose Vinicius Da Silva ),( Kleber Thiago De Oliveira ),( Bianca Ferreira Da Silva ),( Edson Rodrigues Fo ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.6
The oxidative potential of the fungus Penicillium brasilianum, a strain isolated as an endophyte from a Meliaceae plant (Melia azedarach), was investigated using 1-indanone as a substrate to track the production of monooxygenases. The fungus produced the dihydrocoumarin from 1-indanone with the classical Baeyer-Villiger reaction regiochemistry, and (-)-(R)-3-hydroxy-1-indanone with 78% ee. Minor compounds resulting from lipase and SAM activities were also detected. The biotransformation procedures were also applied to a collection of Penicillium and Aspergillus fungi obtained from M. azedarach and Murraya paniculata. The results showed that Baeyer-Villiger were mostly active in fungi isolated from M. azedarach. Almost all of the fungi tested produced 3-hydroxy-1-indanone.
Endophytic Fungi as a Source of Biofuel Precursors
( Santos Fo Florisvaldo C. ),( Taicia Pacheco Fill ),( Joanita Nakamura ),( Marcos Roberto Monteiro ),( Edson Rodrigues Fo ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.7
Endophytic fungi, isolated from a number of different species of tropical plants, were investigated for lipid biodiesel precursor production. The extracts produced from liquid cultures of these fungi were subjected to acidcatalyzed transesterification reactions with methanol producing methyl esters and then analyzed through chromatographic (GC-FID) and spectrometric techniques (MS, NMR ¹H). The European Standard Method, EN 14103, was used for the quantification of methyl esters extracted from the fungi of the species and genera studied. Xylariaceous fungi exhibited the highest concentrations of methyl esters (91%), and hence may be a promising source for biofuel.