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- 주제어 : agro-morphological marker (검색결과 3 건)
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Genetic Diversity Based on Morphology and RAPD Analysis in Vegetable Soybean
A. K. Chowdhury,P. Srinives,P. Tongpamnak,P. Saksoong 韓國作物學會 2001 Korean journal of crop science Vol.46 No.2
Genetic diversity of 47 East-Asian vegetable soybean was characterized by means of agro-morphological traits and RAPD markers. A field trial was conducted to evaluate 14 agro-morphological traits. To study RAPD-based DNA analysis, a total of sixty 10-mer random primers were screened. Of these, 23 polymorphic markers in 16 varieties used for screening. Among 207 markers amplified, 48 were polymorphic for at least one pairwise comparison within the 47 varieties. A higher differentiation level between varieties was observed by using RAPD markers compared to morphological markers. Correspondence analysis using both types of marker showed that RAPD data could fully discriminate between all varieties, whereas morphological markers could not achieve a complete discrimination. Genetic distances between the varieties were estimated from simple matching coefficients, ranged from 0.0 to 0.640 with an average of 0.295~pm 0.131 for morphological traits and 0.042 to 0.625 with an average of 0.336~pm 0.099 for RAPD data, respectively. Cluster analysis based on genetic dissimilarity of these varieties gave rise to 4 distinct groups. The clustering results based on RAPDs did not match with those based on morphological traits. Geographical distribution of most varieties in each of the groups were not well defined. The results suggested that the level of genetic diversity within this group of East-Asian vegetable soybean varieties was sufficient for a breeding program and can be used to establish genetic relationships among them with unknown or unrelated pedigrees.
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Genetic Diversity Based on Morphology and RAPD Analysis in Vegetable Soybean
Srinives, P.,Chowdhury, A.K.,Tongpamnak, P.,Saksoong, P. The Korean Society of Crop Science 2001 Korean journal of crop science Vol.46 No.2
Genetic diversity of 47 East-Asian vegetable soybean was characterized by means of agro-morphological traits and RAPD markers. A field trial was conducted to evaluate 14 agro-morphological traits. To study RAPD-based DNA analysis, a total of sixty 10-mer random primers were screened. Of these, 23 polymorphic markers in 16 varieties used for screening. Among 207 markers amplified, 48 were polymorphic for at least one pairwise comparison within the 47 varieties. A higher differentiation level between varieties was observed by using RAPD markers compared to morphological markers. Correspondence analysis using both types of marker showed that RAPD data could fully discriminate between all varieties, whereas morphological markers could not achieve a complete discrimination. Genetic distances between the varieties were estimated from simple matching coefficients, ranged from 0.0 to 0.640 with an average of 0.295$\pm$0.131 for morphological traits and 0.042 to 0.625 with an average of 0.336$\pm$0.099 for RAPD data, respectively. Cluster analysis based on genetic dissimilarity of these varieties gave rise to 4 distinct groups. The clustering results based on RAPDs did not match with those based on morphological traits. Geographical distribution of most varieties in each of the groups were not well defined. The results suggested that the level of genetic diversity within this group of East-Asian vegetable soybean varieties was sufficient for a breeding program and can be used to establish genetic relationships among them with unknown or unrelated pedigrees.
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( Bahaa El-dein El-sayd Abd El-fatah ) 한국육종학회 2018 Plant Breeding and Biotechnology Vol.6 No.3
Five concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and three combinations of indole-3-acetic acid (IAA) and benzyl-amino purine (BA) were used to analyze response to mature-embryo culture for six genotypes of wheat (Triticum aestivum L.) The differences between genotypes as well as between the concentrations of hormones were significant for three tissue culture traits, namely callus formation (C.%), embryogenic calli (E.C.%) and plant regeneration per embryogenic callus (No. Sh/E.C.). Murashige and Skoog basal medium (MS) supplemented with 7 mg/L 2,4-D showed the highest percentage of callus formation while the highest percentage of embryogenic calli (% of E.C.) was obtained on MS medium supplemented with 4 mg/L 2,4-D. MS medium containing 0.1 mg/L IAA and 1 mg/L BA exhibited the highest number of plant regeneration (No. Sh/E.C.). A half diallel mating was designed to produce 15 crosses among six genotypes. Mean square of the diallel analysis of variance indicated highly significant differences among the parents and F1 hybrids for E.C.% and No. Sh/E.C. traits. The results revealed that Lin-6 (P4) was the best combiner for E.C.% and No. Sh/E.C. Estimates of narrow-sense heritability were 83.1% and 69.0% for E.C.% and No. Sh/E.C., respectively. The genetic diversity among six wheat genotypes was analyzed using three tissue culture traits, 10 agro-morphological traits and three molecular marker systems. The Mantel test showed a positive and significant correlation between tissue culture traits studied and each of agro-morphological traits and molecular marker systems.
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