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RISS 인기검색어
- 검색키워드
- 주제어 : Repellency bioassay (검색결과 3 건)
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Cheng Zuo-Hui,Fan Fang-Fang,Zhao Jin-Zhong,Li Rui,Li Sheng-Cai,Zhang En-Jia,Liu Yu-Kun,Wang Jue-Ying,Zhu Xiang-Run,Tian Yong-Ming 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
The microemulsion formulation (hereafter formulation) of curcuma oil and its acaricidal efficacy against Tetranychus cinnabarinus Boisduval (Acari: Tetranychidae) were optimized in the laboratory to evaluate their spray effectiveness of oviposition inhibition and repellence. Ethovision XT6 was used to analyse the effects of the sublethal concentrations (LC 20 ) of curcuma oil and the formulation on the behaviors of T. cinnabarinus. The results showed that Tween-80 was the best surfactant, Isopropanol was the best co-surfactant and K m = 2:1 was the best condition for the formulation. The prepared microemulsions are stable under conditions of centrifugation and incubation for extended periods. The results showed that the effect of the spray bioassays of the formulation against T. cinnabarinus continuously increased during the experiment, but for curcuma oil almost no longer increase observed when the exposure time went beyond 24 h. Moreover, compared with curcuma oil (LC 50 = 0.716%), the spray bioassay of the formulation (LC 50 = 0.035%) was stronger against T. cinnabarinus. The repellency of the formulation to T. cinnabarinus was stronger with increasing exposure time, but that of curcuma oil declined after 12 h of exposure. The mobile distance of T. cinnabarinus treated with the formulation continuously declined during the experiment but that due to the curcuma oil almost no longer declined when the treatment time reached 12 h. The maximum mobile frequency of T. cinnabarinus treated by curcuma oil and the formulation was recorded at 6 h and 12 h, respectively. Thus, the formulation is a promising candidate as a botanical acaricide of green vegetables.
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A bioassay for natural insect repellents
Eddie Hang Chio,En-Cheng Yang 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.4
A practical bioassay for natural product insect repellents was developed and validated. The Asian tiger mosquito (Aedes albopictus) was used in this study. Djulis (Chenopodium spp.), an indigenous plant from Taiwan, provided better insect repellency than neem tree (Azadirachta indica) oil, a well-known insect repellent. The % repellency of each test material was converted to 50% effective dosage (ED50) by probit analysis for better comparison between test materials. The ED50 for insect repellency in descending order was djulis leave extract (0.532%), neem oil (0.579%); djulis seed extract (0.930%) and sea lily (Crinoid spp.) (1.022%). A practical bioassay for natural product insect repellents was developed and validated. The Asian tiger mosquito (Aedes albopictus) was used in this study. Djulis (Chenopodium spp.), an indigenous plant from Taiwan, provided better insect repellency than neem tree (Azadirachta indica) oil, a well-known insect repellent. The % repellency of each test material was converted to 50% effective dosage (ED50) by probit analysis for better comparison between test materials. The ED50 for insect repellency in descending order was djulis leave extract (0.532%), neem oil (0.579%); djulis seed extract (0.930%) and sea lily (Crinoid spp.) (1.022%).
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Manas Sarkar,Ambadas Akulwad,Rajendra Kshirsagar 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
The recent advancement in new generation fluorinated pyrethroids (e.g., transfluthrin, metofluthrin etc.), the use of semi-volatile vapour phase insecticides for control of mosquitoes and other domestic pests rises. Enabling the examination of the vapour toxicity profiles of these molecules and many other similar new generation molecules will provide new avenues for researchers for understanding the bio-potency in the spatial killing of pests. Hence, it is critical to establish a well-controlled portable vapour-phase bioassay method that can provide the desired precision, accuracy, linearity and robustness. In this respect, we have designed a vapour-toxicity apparatus comprising glass assemblies and developed a novel bioassay method. We found that KT 50 and percentage knockdown at 60 min reflect the concentration dependency. This validates and confirms that the method is sensitive enough to distinguish between concentrations and suitable for concentration-response experiments. We found that KT 50 and percentage knockdown at 60 min at a given concentration does not differ significantly between experiments. Hence, the method has repeatability and precision. Percentage mortality and total KT 50 against Culex quinquefasciatus shows that percentage mortality increases and KT 50 decreases linearly with the increasing concentration. This method provides an easy to operate tool to test the vapour toxicity profiles of any vapour phase insecticide molecules against mosquitoes and flying insects.
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