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RISS 인기검색어
- 검색키워드
- 주제어 : Kigelia pinnata (검색결과 2 건)
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Olubunmi Atolani,Gabriel A. Olatunji,Oluwatoyin Adenike Fabiyi,Adekunle J. Adeniji,Omonike O. Ogbole 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.10
Studies suggest that the traditional applications of Kigelia pinnata leaves have beneficial effects against oxidative stress–mediated diseases and cancers. The pulverized dried leaves of K. pinnata were extracted with hexane, ethylacetate, and methanol sequentially, and the crude extracts were fractionated by silica gel column chromatography with solvent gradient of increasing polarity. 3-hydro-4,8-phytene, trans-phytol, (9Z,12Z)-methyl octadeca-9,12-dienoate, and two oil fractions were obtained. The chemical compositions of chromatographic fractions were determined using gas chromatography–mass spectroscopy. The structure elucidations of the isolated compounds were based on FTIR, MS, and NMR spectral data analyses. These along with the crude extracts were examined for their antioxidant activities using ferric reducing antioxidant power (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS) assays. Total phenolic contents were also determined. The crude extracts and purified compounds were evaluated on the rhabdomyosarcoma human cancer cell for their cytotoxicity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assays. The methanol extract was richer in phenolics and was most potent as antioxidant and cytotoxic agent among all the substances tested. Among the fractions and pure compounds, the two oil fractions showed more cytotoxicity potency, with IC50s of 143.4 – 0.5 and 147.9 – 1.3 ng/mL, which is more significant than the reference standard, cyclophosphamide (165.6 – 1.0 ng/mL). 3-hydro-4,8-phytene showed lower antioxidant and cytotoxicity potential (IC50 = 1818 – 5.2 lg/mL and 171.7 – 0.8 ng/mL, respectively). Trans-phytol did not show a high cytotoxic power (IC50 =769.8 – 4.3 ng/mL). The comparatively high cytotoxicity index of (9Z, 12Z)-methyl octadeca-9,12-dienoate (IC50 = 153.3 –0.1 ng/mL) indicated that it may be one of the principal cytotoxic agent in the ethyl acetate extract. These results suggest that the leaves of K. pinnata possess tumor cytotoxic potential and could be part of a drug combination for future cancer chemotherapy.
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High Frequency In Vitro Regeneration of Kigelia pinnata L. Via Organogenesis
Puthur, Jos T.,Thomas, T. Dennis 한국식물학회 2004 Journal of Plant Biology Vol.47 No.1
An effective and reproducible protocol for the micropropagation of Kigelia pinnata L through high frequency callus regeneration is described. Seeds were surface sterilized before culturing on Gamborgs basal medium (B5 medium). After two weeks the cotyledonary node along with a portion of the hypocotyl were carefully excised from well-developed embryos and subcultured on B5 medium supplemented with different concentrations of 2,4-D and BAP. The cultured cotyledonary node explants showed callus formation at the base of the lower cut end of the hypocotyl. This callus showed shoot initiation after two weeks of subculture on the regeneration medium supplemented with various concentrations of BAP alone or in combination with NAA. The highest number of shoot regeneration occurred on medium containing 5 μM BAP and 0.1 μM NAA. The optimum rooting of the regenerated shoots was observed on %B5 medium supplemented with 4 μM IBA. Micropropagated plants were successfully established in soil in field condition with a survival frequency of 100%.
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