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      • KCI등재후보

        陸地棉과 亞細亞棉의 種間交雜에서 花粉發芽, 受精 및 種子 發育

        Hong Jae Park(朴洪在),Kyung Soo Min(閔庚洙),Jang Soon Ahn(安奬淳) 한국육종학회 1997 한국육종학회지 Vol.29 No.1

        Histological studies were carried out to investigate a period of pollen germination, pollen tube elongation, fertilization, endosperm and embryo development and fiber formation in Gossypium hirsutum, G. arboreum and their reciprocal crosses. Pollen germination started within 30 minutes after pollination in the selfings of G. hirsutum and G. arboreum, and G. arboreum×G. hirsutum and cross, but after 1 hour in G. hirsutum×G. arboreum cross. The fertilization of the egg took 24~30 hours in G. hirsutum selfings, 28~30 hours in G. arboreum selfings, 36~44 hours in G. hirsutum×G. arboreum cross and 28~36 hours in G. arboreum×G. hirsutum cross. Proembryos appeared 5 days after pollination in the selfings and embryos started to abort 10 days after pollination in the interspecific crossing. In the testa fiber, fiber cell began to grow in 24~48 hours after pollination, length of a matured fiber was 22~32mm and 19~22mm in Gossypium hirsutum and G. arboreum, respectively. The development of testa fiber was stopped to grow 7~10 days after pollination in Gossypium hirsutum×G. arboreum, and the length of fiber was 10~13mm. The ovule and embryo culture to obtain the interspecific hybrid plants between Gossypium hirsutum and G. arboreum should start 2 to 6 days after pollination, when endosperm degeneration initiates.

      • KCI등재

        Brief bioinformatics identification of cotton bZIP transcription factors family from Gossypium hirsutum, Gossypium arboreum and Gossypium raimondii

        Khanale Vaishali,Bhattacharya Anjanabha,Satpute Rajendra,Char Bharat 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.4

        Key message Transcription factors (TFs) are the key regulators in plant development and stress adaptation. Under- standing interactions of TFs in cotton crop is important for enhancing stress tolerance and yield enhancement. Among plant TFs, bZIPs plays a major role in seed germination, flower development, biotic and abiotic stress response. Most of the bZIP proteins from cotton remains uncharacterized and can be utilized for crop improvement. Bioinformatics analysis of bZIPs of cultivated cotton species G. hirsutum along with two sub-genome species G. arboreum and G. raimondii at one platform will certainly help the researchers in the selection of specific cotton bZIP genes according to the close alignment with Arabidopsis orthologs or sub-genome homolog for functional characterization. Abstract Cotton is an important commodity in the world economy. Transcription factors (TFs) are the key regulators in plant development and stress adaptation. Understanding interactions of TFs in cotton crop is important for enhancing stress tolerance and yield enhancement. Among plant TFs, bZIPs plays a major role in seed germination, flower development, biotic and abiotic stress response. Most of the bZIP proteins from cotton remains uncharacterized and can be utilized for crop improvement. In this study, we have carried out genome-wide identification and bioinformatics characterization of basic leu- cine zipper domain proteins (bZIPs) from cultivated cotton species Gossypium hirsutum along with two sub-genome species of allotetraploid cotton, Gossypium arboreum and Gossypium raimondii. A total of 228 bZIP genes of G. hirsutum, 91 bZIP genes of G. arboreum and 86 bZIP genes of G. raimondii were identified from CottonGen database. Cotton bZIP genes were annotated in standard pattern according to their match with Arabidopsis bZIPs. Multiple genes with similar bZIP designa- tions were observed in cotton, linked to the gene duplication. Cotton bZIPs are distributed across all 13 chromosomes with varied density. Phylogenetic characterization of all three cotton species bZIPs classified them into 12 subfamilies, namely A, B, C, D, E, F, G, H, I, J, K and S and further into eight subgroups according to their predicted functional similarities, viz., A1, A2, A3, C1, C2, S1, S2 and S3. Subfamilies A and S are having maximum number of bZIP genes, subfamilies B, H, J and K are single-member families. Cotton bZIP protein functions were predicted from identified motifs and orthologs from varied species. Basic region leucine zipper (BRLZ) domain analysis of G. raimondii bZIPs revealed the presence of conserved basic region motif N-X7-R/K in almost all subfamily members, variants are GrbZIP62 with N-X7-I motif and GrbZIP76 with K-X7-R motif. Leucine heptad repeats motif, are also present in variant numbers from two to nine with leucine or other hydrophobic amino acid at designated position among 12 subfamily members. STRING protein interaction network analysis of G. raimondii bZIPs observed strong interaction between A and D, B and K, and C and S subfamily members.

      • KCI등재후보

        陸地棉, 亞細亞棉 및 種間雜種의 胚珠培養

        Hong Jae Park(朴洪在),Kyung Soo Min(閔庚洙),Yong Bee Oh(吳龍飛),Dong Hee Chung(鄭東熙) 한국육종학회 1996 한국육종학회지 Vol.28 No.4

        This experiments were conducted to establish ovule culture method for Gossypium hirsutum, G. arboreum and their hybrids to overcome difficulty to interspecific hybridization due to the ovule abortion in artificial crosses. In the ovule culture of Gossypium hirsutum and G. arboreum the percentage of survival ovule and callus formation in four weeks after culture was 45.6% in G. hirsutum and 37.3% in G. arboreum using MS medium including casein hydrolysate 250㎎/ℓ, IAA 5.0μM and kinetin 0.2μM concentration. The Schenk-Hildebrandt medium was superior to Beasley-Ting or Murashige-Skook ones for ovule culture of interspecific hybrid. Schenck-Hildebrandt medium added by LAA 5μM, Kinetin 0.2μM concentration showed the highest in the rate of survival embryo and plant formation with 44.4% and 2.9% in G. hirsutum×G. arboreum hybrids and 20.1%, 1.5% in the reciprocal hybrids, respectively. Both cotton species showed higher embryo survival and shoot formation in ovule culture (SH medium, 28℃) under the first fifteen days dark and then 16 hours condition light/8 hours dark conditions compared with continuous dark or light treatments. The hybrid ovules dissected out at four days after interspecific pollinations revealed high embryo survival and shoot formation.

      • KCI등재후보

        陸地棉과 亞細亞棉의 種間交配時 生長調節劑 處理가 着삭에 미치는 影響

        Hong Jae Park(朴洪在),Kyung Soo Min(閔庚洙),Dong Hee Chung(鄭東熙) 한국육종학회 1996 한국육종학회지 Vol.28 No.3

        The present cotton cultivars were bred mostly for spinning. Accordingly their fibers are not satisfactory in elasticity, strength and water absorption, which are very important properties when they are used as padding or sanitary purposes. Interspecific hybridization was carried out with a hope to incorporate the high yield potential and early maturity of upland cottons, Gossypium hirsutum, with the highly elastic and strong fibers and excellent water absorption of Asiatic cottons, G. arboreum. Various methods such as application of growth regulators to the pollinated flowers, pollination on cut stems, pasting the stigma with pollen germination media, in vitro culture of hybrid ovules and doubling the chromosomes of the F₁ hybrid plants were attempted to overcome the interspecific barriers. An aqueous solution of 100㎎/ℓ NAA and 50㎎/ℓ GA₃ applied to the interspecifically pollinated flowers and then substantially delayed the fruit drops as shown by 49.5% bolls retained in 20 days after pollination compared to 10.9% in the control. Number of seeds harvested per interspecific cross was 0.017 on G. hirsutum and 0.008 on G. arboreum, even through all of them were empty and failed to germinate.

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