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Kang, Dong-Hyun,Han, Won Bae,Choi, Nakwon,Kim, Yong-Jun,Kim, Tae Song American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.47
<P>Artificial lipid membranes are excellent candidates for new biosensing platforms because their structures are similar to cell membranes and it is relatively easy to modify the composition of the membrane. The freestanding structure is preferable for this purpose because of the more manageable reconstitution of the membrane protein. Therefore, most of the lipid membranes for biosensing are based on two-dimensional structures that are fixed on a solid substrate (unlike floating liposomes) even though they have some disadvantages, such as low stability, small surface area, and potential retention of solvent in the membrane. In this paper, three-dimensional freestanding lipid bilayer (3D FLB) arrays were fabricated uniformly on SU-8 microwells without any toxic solvent. The 3D FLBs have better stability and larger surface area due to their cell-like structure. In order to improve the sealing characteristics of the 3D FLBs, the applied frequency of the ac field was controlled during the electroformation. The 3D FLBs were observed through transparent SU-8 microwell arrays using confocal microscopy and demonstrated perfect sealing until 5.5 days after the electroformation at more than 1 kHz. Also, the details of the sealing of a fixed 3D freestanding lipid structure were discussed for the first time. The unilamellarity and biofunctionality of the 3D FLBs were verified by a transport protein (α-hemolysin) assay.</P> [FIG OMISSION]</BR>
Monatomic Chemical-Vapor-Deposited Graphene Membranes Bridge a Half-Millimeter-Scale Gap
Lee, Choong-Kwang,Hwangbo, Yun,Kim, Sang-Min,Lee, Seoung-Ki,Lee, Seung-Mo,Kim, Seong-Su,Kim, Kwang-Seop,Lee, Hak-Joo,Choi, Byung-Ik,Song, Chang-Kyu,Ahn, Jong-Hyun,Kim, Jae-Hyun American Chemical Society 2014 ACS NANO Vol.8 No.3
<P>One of the main concerns in nanotechnology is the utilization of nanomaterials in macroscopic applications without losing their extreme properties. In an effort to bridge the gap between the nano- and macroscales, we propose a clever fabrication method, the inverted floating method (IFM), for preparing freestanding chemical-vapor-deposited (CVD) graphene membranes. These freestanding membranes were then successfully suspended over a gap a half-millimeter in diameter. To understand the working principle of IFM, high-speed photography and white light interferometry were used to characterize and analyze the deformation behaviors of the freestanding graphene membranes in contact with a liquid during fabrication. Some nanoscale configurations in the macroscopic graphene membranes were able to be characterized by simple optical microscopy. The proposed IFM is a powerful approach to investigating the macroscopic structures of CVD graphene and enables the exploitation of freestanding CVD graphene for device applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2014/ancac3.2014.8.issue-3/nn405805s/production/images/medium/nn-2013-05805s_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn405805s'>ACS Electronic Supporting Info</A></P>
Thin and large free-standing PDMS membrane by using polystyrene Petri dish
채수경,Ji-Hee Ryoo,이상훈 한국바이오칩학회 2012 BioChip Journal Vol.6 No.2
In this technical report, we describe the use of a polystyrene Petri dish to fabricate thin, freestanding polydimethylsiloxane (PDMS) membranes without using a sacrificial layer. We hypothesized that the low work of adhesion between the membrane and the substrate enabled the easy separation of large area thin membranes without extra treatment. To demonstrate this principle, we prepared four substrates with different surface properties: a bare silicon (Si) wafer, a polystyrene (PS) Petri dish, a Si wafer coated with SU-8, and a Si wafer coated with an AZ1512 sacrificial layer. The PDMS had the lowest work of adhesion to the PS Petri dish, which was verified by the Harmonic mean method. Unlike the others three substrates, the thin PDMS membrane on the PS Petri dish could be easily separated without the need for organic solvents and a sacrificial layer. The thickness of produced membrane was measured by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Also, we demonstrated that the high stretching property of thin and large free-standing PDMS membranes enabled the production of arrayed diverse micro-patterned 3D curved structures with a high aspect ratio.