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x110,ox161,ić,, Marija,Eraković,, Sanja,Janković,, Ana,Vukax161,inović,-Sekulić,, Maja,Matić,, Ivana Z.,Stojanović,, Jovica,Rhee, Kyong Yop,Miš,ković Elsevier 2017 Journal of industrial and engineering chemistry Vol.47 No.-
<P><B>Abstract</B></P> <P>Graphene (Gr) and natural polymer chitosan (CS) were introduced to hydroxyapatite (HAP) to produce a three-component composite coating, which was fabricated by cathodic electrophoretic deposition on Ti substrates in an ethanol suspension. These HAP/CS/Gr coatings were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS) and electrochemical measurements and found that the graphene into HAP/CS composites significantly improves their morphology, thermal stability, and bioactivity. Both HAP/CS and HAP/CS/Gr composite coatings are classified as non-cytotoxic when tested against healthy peripheral blood mononuclear cells (PBMC), while antibacterial activity against <I>Staphylococcus aureus</I> and <I>Escherichia coli</I> could not be verified.</P>
Nex161,ović,, Katarina,Janković,, Ana,Kojić,, Vesna,Vukax161,inović,-Sekulić,, Maja,Perić,-Grujić,, Aleksandra,Rhee, Kyong Yop,Miš,ković,-Stankovi Elsevier 2018 Composites. Part B, Engineering Vol.154 No.-
<P><B>Abstract</B></P> <P>This study presents the synthesis of novel silver/poly(vinyl alcohol)/chitosan/graphene (Ag/PVA/CHI/Gr) nanocomposite hydrogels by <I>in situ</I> electrochemical reduction of silver ions in the hydrogel matrix and their thorough characterization by UV–visible and Raman spectroscopies, field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and silver release measurements. The influence of chitosan content on the incorporation and stabilization of silver nanoparticles (AgNPs) was investigated, and we found that hydrogels with higher chitosan content contain higher amounts of AgNPs. In addition, the cytotoxicity and antibacterial activity of Ag/PVA/CHI/Gr nanocomposite hydrogels were evaluated. Based on <I>in vitro</I> investigations, the obtained materials exhibit diffusion-controlled release profiles over 28 days, strong antibacterial activity against <I>Staphylococcus aureus</I> and <I>Escherichia coli</I> bacterial strains, and no cytotoxicity toward human and mice fibroblast cell lines.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>In situ</I> electrochemical synthesis of silver nanoparticles in PVA/CHI/Gr hydrogels. </LI> <LI> Effect of chitosan concentration on the amount of embedded silver nanoparticles. </LI> <LI> Effect of chitosan concentration on the silver release kinetics. </LI> <LI> Strong antibacterial activity against <I>S. aureus</I> and <I>E. coli</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Janković,, Ana,Eraković,, Sanja,Vukax161,inović,-Sekulić,, Maja,Miš,ković,-Stanković,, Vesna,Park, Soo Jin,Rhee, Kyong Yop Elsevier 2015 Progress in organic coatings Vol.83 No.-
<P><B>Abstract</B></P> <P>The graphene based silver/hydroxyapatite/graphene (Ag/HAP/Gr) composite coatings were produced by electrophoretic deposition (EPD) on titanium to assemble porous bioactive homogenous coatings, with improved corrosion stability in simulated body fluid (SBF). Novel composite coatings were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), Raman spectroscopy, X-ray photoelectron analysis (XPS) and electrochemical impedance spectroscopy (EIS). The improved properties of graphene based coatings in respect to the graphene-free coatings, as a consequence of toughening action of graphene, were demonstrated: reduced surface cracks, better mechanical resistance (hardness and elastic modulus increased by 10%) and enhanced thermal stability, while the Ca/P ratio was closer to the stoichiometric value. The bioactivity of Ag/HAP/Gr composite coatings was proved by a newly formed apatite layer in SBF with enhanced corrosion stability, while antibacterial activity against <I>Staphylococcus aureus</I> and <I>Escherichia coli</I> and noncytotoxicity against healthy peripheral blood mononuclear cells (PBMC) indicate the high potential for biomedical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel graphene based composite coating on titanium obtained by electrophoretic deposition. </LI> <LI> Homogenous, non-toxic Ag/HAP/Gr coating with improved mechanical properties. </LI> <LI> New apatite layer formation in SBF has proved the coating bioactivity. </LI> <LI> Diminished bacterial growth of <I>S. aureus</I> and <I>E. coli</I> after only 3h of exposure. </LI> </UL> </P>