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Martins-Filho Paulo Ricardo,dos Santos Joyce Thayane da Conceição,Rezende Márcia Santos,de Carvalho Fernanda Oliveira,dos Reis Érica Santos,Barboza Waneska de Souza,Cavalcante Taise Ferreira,dos Sant 한국역학회 2023 Epidemiology and Health Vol.45 No.-
This study estimated the prevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in urban cleaning and solid waste management workers during the transmission of the Omicron variant in one of the poorest regions of Brazil (the state of Sergipe). Nasopharyngeal swabs were collected from 494 workers, and the presence of SARS-CoV-2 RNA was tested by quantitative reverse-transcriptase polymerase chain reaction. Data on sociodemographic characteristics, comorbidities, vaccination status, mask use, and use of public transport to commute to the workplace were collected. The prevalence with a 95% confidence interval (CI) was calculated from the proportion of SARS-CoV-2 positive cases among the total number of individuals tested. The prevalence ratio (PR) with a 95% CI was the measure of association used to evaluate the relationship between SARS-CoV-2 infection and the exposure variables. The prevalence of SARS-CoV-2 infection was 22.5% (95% CI, 19.0%–26.4%). Individuals under the age of 40 had a higher prevalence of infection (PR=1.53; 95% CI, 1.03–2.30) as well as those who did not believe in the protective effect of vaccines (PR=1.78; 95% CI, 1.05–2.89). Our results indicate the need for better guidance on preventive measures against COVID-19 among urban cleaning and solid waste management workers.
Danielle Kian,Cesar Armando Contreras Lanche,Igor Alexandre Campos Damiani,Tamiris Zanforlin Olmos Fernan,Phileno Pinge-Filho,Marcia Regina Machado dos Sant,Jose Franco da Silveira,Celso Vataru Nakamu 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.4
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.