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Garbary, David J.,Zuchang, Pei The Korean Society of Phycology 2006 ALGAE Vol.21 No.3
Mitochondrial distribution and abundance were assessed during the growth of apical and subapical cells in the red algae Colaconema caespitosum (J. Agardh) Jackelman, Stegenga and Bolton and Antithamnion cruciatum (C. Agardh) Nägeli after staining with 3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] and 2,4’-dimethylaminostyryl-Nethylpyridinium iodide (DASPEI). In fully elongate apical cells of C. caespitosum there were 100-120 mitochondria. During apical cell enlargement and division there is a doubling and then halving of the mitochondrial numbers. Apical cells prior to cytokinesis in young filaments are smaller than in mature filaments (ca. 50 and 100 μm long, respectively) and have fewer mitochondria (ca. 100 and 120 mitochondria per cell, respectively). In older vegetative cells mitochondria tend to aggregate at opposite ends of the cells with some mitochondria associated with the central nucleus or at points of apparent branch initiation. There is a greater density of mitochondria in apical cells of smaller versus larger plants (one mitochondrion per 6.3 μm3 and 9.8 μm3, respectively), suggesting that apical cells of younger plants may be more metabolically active. Male and female gametophytic thalli of Antithamnion cruciatum had similar numbers of mitochondria in apical cells of indeterminate axes, as did gametophytic and sporophytic thalli. There were about 40-50 mitochondria in fully elongated apical cells with about half this number in newly divided apical and subapical cells. Apical cells of determinate branches had more mitochondria (60-77) than indeterminate branches (60-70 vs. 40-50). In both species and in all cell types mitochondrial numbers were highly correlated with cell size.
Tongue Image Segmentation via Thresholding and Gray Projection
( Weixia Liu ),( Jinmei Hu ),( Zuoyong Li ),( Zuchang Zhang ),( Zhongli Ma ),( Daoqiang Zhang ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.2
Tongue diagnosis is one of the most important diagnostic methods in Traditional Chinese Medicine (TCM). Tongue image segmentation aims to extract the image object (i.e., tongue body), which plays a key role in the process of manufacturing an automated tongue diagnosis system. It is still challenging, because there exists the personal diversity in tongue appearances such as size, shape, and color. This paper proposes an innovative segmentation method that uses image thresholding, gray projection and active contour model (ACM). Specifically, an initial object region is first extracted by performing image thresholding in HSI (i.e., Hue Saturation Intensity) color space, and subsequent morphological operations. Then, a gray projection technique is used to determine the upper bound of the tongue body root for refining the initial object region. Finally, the contour of the refined object region is smoothed by ACM. Experimental results on a dataset composed of 100 color tongue images showed that the proposed method obtained more accurate segmentation results than other available state-of-the-art methods.