In vitro antibacterial activity of flavonoids extracts from three Algerian horehound (Marrubium vulgare L.) leaves

Karim Bouterfas,Zoheir Mehdadi,Manal Maliha Elaoufi,Linda Aouad,Ali Latreche,Walid Benchiha 경희대학교 융합한의과학연구소 2018 Oriental Pharmacy and Experimental Medicine Vol.18 No.1

Marrubium vulgare L. is used in folk medicine of several countries. Many researches showed that it is used for treatment of variety of diseases, for example as remedy for asthma and diabetes, duo to its antibacterial effects. The present study aimed to access the effect of the sampling location on the antibacterial activity of the flavonoids extracted from Marrubium vulgare L. leaves, against three pathogenic bacteria; Escherichia coli, Bacillus cereus, and Proteus mirabilis. The leaves were collected from three different sampling locations belonging northwest Algeria: Tessala Mountain, M’sila forest and Ain Skhouna. The flavonoid extraction was carried out using organic solvents with increasing polarity. The bacterial susceptibility testing by diffusion agar method showed that the diameters zones varied significantly (P < 0.001) according to the sampling location of the leaves, the flavonoid extract and its concentration. The inhibition diameters varied between 7.5–34.3 mm, which often exceed those induced by the standard antibacterial agents (chloramphenicol, gentamicin, aztreonam, nalidixic acid, ceftazidime, and imipenem). The agar dilution method showed that the minimum inhibitory concentrations (MICs) range between 25 and 100 μg/mL; revealing strong antibacterial inhibition. The phytochemical screening revealed the presence of flavans and flavanols, which may be responsible of this promising antimicrobial activity.

Fabrication of Non-enzymatic Electrochemical Glucose Sensor Based on Nano-copper Oxide Micro Hollow-spheres

Zahra Haghparas,Zoheir Kordrostami,Mohsen Sorouri,Maryam Rajabzadeh,Reza Khalifeh 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.4

In the present study, an electrochemical glucose biosensor has been developed based on nano-copper oxide micro hollow spheres. The nano-copper oxide micro hollow spheres were synthesized via hydrothermal method using pluronic F-127 as a surfactant. For structural characterization of CuO hollow sphere structures, the scanning electron microscopy and X-ray diffraction spectroscopy were applied. The performance parameters of the sensor were improved by optimizing the modification process. The electrochemical characteristics of the proposed glucose biosensor were investigated by using cyclic voltammetry and chronoamperometry techniques in both 0.1 M and 1M NaOH solutions. The results revealed that the proposed electrode has a wide dynamic range from 1 μM to 11.50 mM for glucose detection at 0.1 M NaOH solution which covers two linear ranges from 1 μM to 3 mM and from 3 mM to 11.50 mM. The sensitivities of the two linear ranges were obtained as 25.0 ± 0.8 μA·mM-1·cm-2 and 13.6 ± 0.3 μA·mM-1·cm-2, respectively. An extremely wide linear range from 1 μM to 16 mM with a sensitivity of 35.2 ± 0.4 μA·mM-1·cm-2 was achieved for the 1 M NaOH solution. The sensor achieved a 1 μM practical lowest limit of detection which is an excellent low limit of detection at both NaOH concentrations compared to some important previously reported works. In addition, the good tolerance toward the interfering species and the satisfactory behavior in real sample analysis verified the promising performance of the proposed sensor.

Nano-Micelle of Moringa Oleifera Seed Oil Triggers Mitochondrial Cancer Cell Apoptosis

Abd-Rabou, Ahmed A,Zoheir, Khairy M A,Kishta, Mohamed S,Shalby, Aziza B,Ezzo, Mohamed I Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.11

Cancer, a worldwide epidemic disease with diverse origins, involves abnormal cell growth with the potential to invade other parts of the body. Globally, it is the main cause of mortality and morbidity. To overcome the drawbacks of the commercially available chemotherapies, natural products-loaded nano-composites are recommended to improve cancer targetability and decrease the harmful impact on normal cells. This study aimed at exploring the anti-cancer impacts of Moringa oleifera seed oil in its free- (MO) and nano-formulations (MOn) through studying whether it mechanistically promotes mitochondrial apoptosis-mediating cell death. Mitochondrial-based cytotoxicity and flow cytometric-based apoptosis analyses were performed on cancer HepG2, MCF7, HCT 116, and Caco-2 cell lines against normal kidney BHK-21 cell line. The present study resulted that MOn triggered colorectal cancer Caco-2 and HCT 116 cytotoxicity via mitochondrial dysfunction more powerful than its free counterpart (MO). On the other side, MOn and MO remarkably induces HCT 116 mitochondrial apoptosis, while sparing normal BHK-21 cells with minimal cytotoxic effect. The present results concluded that nano-micelle of Moringa oleifera seed oil (MOn) can provide a novel therapeutic approach for colorectal and breast cancers via mitochondrial-mediated apoptosis, while sparing normal and even liver cancer cells a bit healthy or with minimal harmful effect. Intriguingly, MOn induced breast cancer not hepatocellular carcinoma cell death.

Diagnostic value of two modes of cone-beam computed tomography in evaluation of simulated external root resorption: an in vitro study

Dalili, Zahra,Taramsari, Mehran,Mehr, Seyed Zoheir Mousavi,Salamat, Fatemeh Korean Academy of Oral and Maxillofacial Radiology 2012 Imaging Science in Dentistry Vol.42 No.1

Purpose : Field of view and voxel resolution of cone beam computed tomography (CBCT) might affect the diagnostic capability. This study was performed to compare between the standard and HiRes zoom modes in the diagnosis of external root resorption (ERR) using CBCT. Materials and Methods : Sixty three small cavities (0.25 mm depth and 0.5 mm diameter) were simulated on the buccal, lingual, and proximal surfaces at three different levels of 16 roots of teeth. After covering the root with nail varnish, the roots were inserted in the sockets and the model was placed in a water-containing lacuna. CBCT scans were taken in both standard and HiRes zoom modes using NewTom VG (QR srl Company, Verona, Italy). Then, an observer assessed the images to determine the presence or absence of the cavities. This process was repeated by increasing the size and depth of cavities to 0.5 mm depth and 1 mm diameter. Data were analyzed by McNemar test. The sensitivity, specificity, positive predictive value, negative predictive value, and likelihood ratio in evaluation of the simulated cavities were calculated. Results : There was a significant difference between the two imaging modes in diagnosing the shallow cavities (p=0.02).The sensitivity of the standard zoom in detecting the shallow cavities was lower than that of the HiRes zoom. The likelihood ratio of the HiRes zoom was higher in the diagnosis of both cavity types. Conclusion : This study suggested that a smaller voxel size in the HiRes zoom mode of CBCT is preferred for diagnosis of ERR.

Diagnostic value of two modes of cone-beam computed tomography in evaluation of simulated external root resorption : An in vitro study

Zahra Dalili,Mehran Taramsari,Seyed Zoheir Mousavi Mehr,Fatemeh Salamat 대한구강악안면방사선학회 2012 Imaging Science in Dentistry Vol.42 No.1

Purpose : Field of view and voxel resolution of cone beam computed tomography (CBCT) might affect the diagnostic capability. This study was performed to compare between the standard and HiRes zoom modes in the diagnosis of external root resorption (ERR) using CBCT. Materials and Methods : Sixty three small cavities (0.25 mm depth and 0.5 mm diameter) were simulated on the buccal, lingual, and proximal surfaces at three different levels of 16 roots of teeth. After covering the root with nail varnish, the roots were inserted in the sockets and the model was placed in a water-containing lacuna. CBCT scans were taken in both standard and HiRes zoom modes using NewTom VG (QR srl Company, Verona, Italy). Then, an observer assessed the images to determine the presence or absence of the cavities. This process was repeated by increasing the size and depth of cavities to 0.5 mm depth and 1 mm diameter. Data were analyzed by McNemar test. The sensitivity, specificity, positive predictive value, negative predictive value, and likelihood ratio in evaluation of the simulated cavities were calculated. Results : There was a significant difference between the two imaging modes in diagnosing the shallow cavities (p=0.02).The sensitivity of the standard zoom in detecting the shallow cavities was lower than that of the HiRes zoom. The likelihood ratio of the HiRes zoom was higher in the diagnosis of both cavity types. Conclusion : This study suggested that a smaller voxel size in the HiRes zoom mode of CBCT is preferred for diagnosis of ERR.