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        QTL Analysis of Floral Traits of Rice (Oryza Sativa L.) under Well-Watered and Drought Stress Conditions

        Hu Songping,Zhou Ying,Zhang Lin,Zhu Xudong,Wang Zhenggong,Li Lin,Luo Lijun,Zhou Qingming 한국유전학회 2009 Genes & Genomics Vol.31 No.2

        Three floral traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES) and dual exserted stigma (PDES) of a RI population with 185 lines under water stress and non-stress conditions for two years were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. High phenotypic correlation was found between PSES and PDES (r = 0.5424***). Based on a linkage map of 203 SSR markers, when under well-watered condition, six QTLs ( qSNP-3b, qSNP-4, qSNP-11 qSNP-2, qSNP-5 and qSNP-9) were detected for SNP. Half of them had significant Q× E interactions. Three QTLs ( qPSES-1, qPSES-2, qPSES-5) were found to influence PSES, including one locus ( qPSES-2) having Q× E interaction. And three QTLs ( qPDES-2, qPDES-5 and qPDES-8) were also detected to influence PDES. qPDES-5 was found to have Q × E interaction. The contribution rate of a single QTL varied from 0.80% to 8.83% for additive effect, and 1.86% to 15.25% for Q × E interactions. Under drought stress, six QTLs ( qSNP-3a, qSNP-4, qSNP-7a, qSNP-7b, qSNP-8 and qSNP-9) were associated with SNP, including qSNP-3a and qSNP-4 with Q × E interaction. Three QTLs ( qPSES-1, qPSES-10 and qPSES-12) were located on rice chromosome 1, 10 and 12 for PSES. Four QTLs ( qPDES-1a, qPDES-1b, qPDES-4 and qPDES-9) were detected for PDES, including qPDES-9 with Q ×E interaction. The additive effect of single QTL can only explain 1.16% to 5.84% of total variance while Q × E interaction of four loci can explain 4.25% to 11.54% of total variance for each locus. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%~9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES and PDES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress conditions, indicating that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion. Three floral traits, spikelet number per panicle (SNP), percentage of single exserted stigma (PSES) and dual exserted stigma (PDES) of a RI population with 185 lines under water stress and non-stress conditions for two years were investigated in a drought tolerance screening facility. ANOVA results showed high significance between years, lines, and water stress treatments, together with interactions among them in pairs. High phenotypic correlation was found between PSES and PDES (r = 0.5424***). Based on a linkage map of 203 SSR markers, when under well-watered condition, six QTLs ( qSNP-3b, qSNP-4, qSNP-11 qSNP-2, qSNP-5 and qSNP-9) were detected for SNP. Half of them had significant Q× E interactions. Three QTLs ( qPSES-1, qPSES-2, qPSES-5) were found to influence PSES, including one locus ( qPSES-2) having Q× E interaction. And three QTLs ( qPDES-2, qPDES-5 and qPDES-8) were also detected to influence PDES. qPDES-5 was found to have Q × E interaction. The contribution rate of a single QTL varied from 0.80% to 8.83% for additive effect, and 1.86% to 15.25% for Q × E interactions. Under drought stress, six QTLs ( qSNP-3a, qSNP-4, qSNP-7a, qSNP-7b, qSNP-8 and qSNP-9) were associated with SNP, including qSNP-3a and qSNP-4 with Q × E interaction. Three QTLs ( qPSES-1, qPSES-10 and qPSES-12) were located on rice chromosome 1, 10 and 12 for PSES. Four QTLs ( qPDES-1a, qPDES-1b, qPDES-4 and qPDES-9) were detected for PDES, including qPDES-9 with Q ×E interaction. The additive effect of single QTL can only explain 1.16% to 5.84% of total variance while Q × E interaction of four loci can explain 4.25% to 11.54% of total variance for each locus. There were one to nine pairs of epistatic QTLs influencing SNP and stigma exsertion. The contribution rates of additive and epistatic effects seemed to be in a low magnitude for most cases (0.76%~9.92%) while a few QTLs or QTL pairs explained more than 10% of total variance. Some main effect QTL and epistasis were commonly detected among PSES and PDES, explaining the high positive correlation between them. Few QTLs were detected under both water stress and non-stress conditions, indicating that drought had severe impact on the genetic behaviors of both spikelet number and stigma exsertion.

      • Homicidal Poisoning of Heroin and Estazolam : Autopsy and Pathological Findings, Toxicological Analysis

        Dan Liu,Shangxun Li,Xiangtao Ma,Jinxue Gao,Liang Xu,Jun He,Yuhong Li,Dan Yan,Yiwu Zhou,Qingming Wu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.4

        We reported an unusual homicidal case in which a 40-year-old woman was deceived into drinking a cup of milk that had 72 tablets of estazolam (2mg/tablet) dissolved in, and then being injected heroin aqueous solution on the right deltoid region by the criminal. At autopsy, pinpoint pupils and a new injection site on the right deltoid region were found. The pathologic pictures showed multiple patchy hemorrhages and considerable amounts of foreign amorphous substance with yellow appearance at the injection site. Some double refracting crystals with the forms of Maltese cross, acicular, rhomb or irregular were found by polarizing microscope, which may result from the diluent in heroin such as starch. Toxicological qualitative analysis by gas chromatography mass spectrometry (GC-MS) demonstrated the presence of benzodiazepine and morphine in blood and urine, and heroin in the injector left at scene. Quantitative analysis was also performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), providing the data on distribution of 6-monoacetylmorphine, morphine and estazolam in the woman’s body. And the cause of death was determined to polydrug heroin-related deaths due to the combined poisoning of heroin and estazolam. It taught a lesson that the determination of other drugs, particularly central nervous system depressants in heroin poisoning were quite important in forensic expertise.

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