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Zhou Yin,Xu Zheng,Chen Xu,Zhou Junjiang,Wang Songtai,Xu Yanqin 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Oriental orchids of the genus Cymbidium are famous for their flower fragrance and have great value in the horticultural market. To investigate the regulatory mechanisms of fragrance biosynthesis in Cymbidium faberi, the promoters of the CfAOC and CfJMT genes in the methyl jasmonate biosynthesis pathway were cloned and analyzed. Notably, the CfAOC promoter was transcriptionally active in all vegetative organs, which was not true for the CfJMT promoter, indicating that the latter is tissue-specific for the flowers of C. faberi. The dual-luciferase assay showed that the fragment between –629 and –1038 nt of the CfAOC promoter, as well as the fragment between –960 and –1121 nt of the CfJMT promoter, displayed specific interaction with the CfbHLH transcription factors. These results provide a theoretical basis for the genetic modification of the flower fragrance of Cymbidium based on specific CfbHLH transcription factors.