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A Comparative Proteomic Analysis of Parthenogenetic Lines and Amphigenetic Lines of Silkworm
Peigang Liu,Yongqiang Wang,Xin Du,Fangxiong Shi,Zhiqi Meng 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4
Parthenogenetic strains of silkworm serve as aneffective system for sex-control in silkworms. To determinethe molecular mechanism of silkworm parthenogenesis,protein profiles from newly hatched silkworm of aparthenogenetic lines with high pigmentation rate andhatching rate were compared with amphigenetic lines usingproteomics approach, including by two-dimensional electrophoresis(2-DE), matrix-assisted laser desorption/ionizationtime-of-flight mass spectrometry (MALDI-TOF/TOF MS),and bioinformatics analysis. Several proteins were expresseddifferentially between the parthenogenetic and amphigeneticlines, and seven of nine interesting proteins were identifiedsuccessfully using MALDI-TOF/TOF MS analysis. Theidentified proteins were muscular protein-20, odorant bindingprotein-LOC100301497, glutathione S-transferase delta,translationally controlled tumor protein homolog, cuticularprotein RR-1 motif 19, beta-actin, actins, and muscle-typeA1 actins. These proteins may be associated with the regulationof growth, development, and reproductive processes ofsilkworm parthenogenetic lines.
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Jine Chen,Baolong Niu,Yongqiang Wang,Yan Liu,Peigang Liu,Zhiqi Meng,Boxiong Zhong 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2
The sex-linked balanced lethal (SLBL) strains of silkworm serve as an effective system for sex-control in silkworm. To gain comprehensive insight into the effect of one sex-linked balanced lethal gene l2, comparative proteomic analysis was carried out between the survival embryos (W +l 1 Z l 1 +l 2 ) and lethal embryos (W +l 1 Z l 1 +l 2 ) before the lethal stage. The lethal stage of l2 was confirmed by observing the typical dead embryo morphology. The two genotype embryos before lethal stage were distinguished using polymorphic simple sequence repeats (SSR) markers closely linked to l2 on the sex chromosome. Finally, 11 differentially expressed protein spots were successfully identified by MALDI-TOF/TOF mass spectrometry (MS). Among them,only 1 protein identified as heat shock protein 20.4(HSP20.4) was up-regulated in the lethal embryos, while the other 10 were down-regulated. The up-regulation of HSP20.4 suggests that there may be abnormal polypeptides produced in the lethal embryos. The gene ontology (GO)annotation indicated those down-regulated proteins are involved in important biological processes including embryo development, nucleoside metabolism, tRNA splicing, translation and protein folding. The biological pathway analysis showed that those down-regulated proteins are mainly involved in spindle assemblage and morphogenesis. Based on our results, we suggest that the l2 may be the mutant expressing abnormal polypeptides. Its expression has a negative effect on mitosis and morphogenesis processes. The death of the embryos may be caused by the accumulation of abnormal polypeptides and the handicap of cell proliferation and morphogenesis.
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