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- 저자 : Zhanghui Dong (검색결과 2 건)
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Zhanghui Dong,Jianhong Zhang,Qingzhu Zhu,Lifen Zhao,Shuxiang Sui,Zengshu Li,Yanli Zhang,Hu Wang,Dongliang Tian,Yankun Zhao 한국유전학회 2018 Genes & Genomics Vol.40 No.3
Drought stress is one of the most important abiotic stresses. Cotton is classified as drought tolerant crop but the regulatory mechanism is unknown. MicroRNAs (miRNAs) have been implicated important roles in stress responses in many plants. However, the study of miRNAs in cotton responsive to drought stress is limited, especially in early-maturing cotton. In this study, we performed deep sequencing of small RNAs to identify known and novel miRNAs involved in the regulation of drought stress and understand the expression profile of miRNAs in early-maturing cotton. Three cotton small RNA libraries: non-stressed Shizao1 (early-maturing cotton variety) library (NSS), drought-stressed Shizao1 library (DSS) and non-stressed Jimian958 (medium-maturing cotton variety) library (NSJ) were constructed for deep sequencing. As a result, we identified a total of 64 known and 67 novel miRNAs in the 3 libraries and 88 of them were dramatically differentially expressed (greater than twofold) during drought stress. In addition, we found the expression of 41 miRNAs increased or reduced more than twofold in early-maturing cotton variety compared with that in medium-maturing cotton variety. Our results significantly increased the number of miRNAs in cotton and revealed for the first time the expression profile of miRNAs for early-maturing cotton.
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Genomic identification of microRNA promoters and their cis-acting elements in Populus
Yanwei Wang,Min Chen,Ming Wei,Zhanghui Dong,Hai Bao 한국유전학회 2016 Genes & Genomics Vol.38 No.4
MicroRNAs (miRNAs) are non-coding singlestranded RNAs of appropriately 21 nucleotides in length that negatively regulate post-transcriptional processes in plants and animals. In recent years, while an increasing number of miRNAs has been identified in various species, little is known regarding the specific transcriptional regulation of miRNAs in Populus. In this investigation, 368 miRNAs belonging to 37 miRNA families in Populus were analyzed to identify promoter regions and predict the distribution patterns of core promoter elements [TATA boxes and transcription start sites (TSSs)], as well as main cisacting elements through a bioinformatics approach. A total of 368 precursor miRNA (pre-miRNA) gene loci belonging to 213 unique miRNAs were detected. We identified 229 promoters and further predicted 121 TSSs and TATA boxes for 139 pre-miRNAs. The conserved motifs, base composition and the average free energy profiles in the neighboring regions of TSSs were further analyzed. In addition, 101 types of cis-acting elements, such as anaerobic induction elements, abscisic acid-response elements, and MYB binding sites, were predicted in the predicted promoter regions of the pre-miRNAs. The data obtained in our investigation may aid in the analysis of specific upstream sequences of pre-miRNAs for differential expression and further our understanding of the molecular biology of miRNAs in Populus.
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