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RISS 인기검색어
- 검색키워드
- 저자 : Yusuke Kawamoto (검색결과 2 건)
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TAMRA-polypyrrole for A/T sequence visualization on DNA molecules
Lee, Seonghyun,Kawamoto, Yusuke,Vaijayanthi, Thangavel,Park, Jihyun,Bae, Jaeyoung,Kim-Ha, Jeongsil,Sugiyama, Hiroshi,Jo, Kyubong Oxford University Press 2018 Nucleic acids research Vol.46 No.18
<P><B>Abstract</B></P><P>Fluorophore-linked, sequence-specific DNA binding reagents can visualize sequence information on a large DNA molecule. In this paper, we synthesized newly designed TAMRA-linked polypyrrole to visualize adenine and thymine base pairs. A fluorescent image of the stained DNA molecule generates an intensity profile based on A/T frequency, revealing a characteristic sequence composition pattern. Computer-aided comparison of this intensity pattern with the genome sequence allowed us to determine the DNA sequence on a visualized DNA molecule from possible intensity profile pattern candidates for a given genome. Moreover, TAMRA-polypyrrole offers robust advantages for single DNA molecule detection: no fluorophore-mediated photocleavage and no structural deformation, since it exhibits a sequence-specific pattern alone without the use of intercalating dyes such as YOYO-1. Accordingly, we were able to identify genomic DNA fragments from <I>Escherichia coli</I> cells by aligning them to the genomic A/T frequency map based on TAMRA-polypyrrole-generated intensity profiles. Furthermore, we showed band and interband patterns of polytene chromosomal DNA stained with TAMRA-polypyrrole because it prefers to bind AT base pairs.</P>
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Victor Lopez-Lopez,Yusuke Ome,Yusuke Kawamoto,Alvaro Gómez Ruiz,Ricardo Robles Campos,Goro Honda 대한내시경복강경외과학회 2020 Journal of Minimally Invasive Surgery Vol.23 No.1
Since the beginning of laparoscopic liver surgery, resection of the posterosuperior segments has been considered one of the most challenging procedure due to its difficult access. The main drawbacks of the laparoscopic approach to dome lesions are poor visualization, the difficulty of instrumentation and the greater complexity in the control of bleeding. In the evolution of minimally invasive techniques from hybrid techniques to the current purely laparoscopic approaches, the different authors have established gradually the currents indications and surgical techniques to operate these segments with a similar feasibility and safety than open approach. The standardization in the patient position, the use of intercostal trocars, the learning curve in laparoscopic liver surgery, the management of the hepatic blood flow and the refinement of the technique in the extrahepatic and intrahepatic Glissonean pedicle approaches, has allowed to leave behind the initial contraindications about the laparoscopic approach in these segments. In the present review of the literature, the accumulated experience of the different groups in minimally invasive liver surgery together with the technological advances in the different laparoscopic devices have facilitated the resection of tumors in segments 7 and 8 with similar and even better results than open surgery.
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